MA5-34742

antibody from Invitrogen Antibodies

Targeting: CENPC CENP-C, CENPC1, hcp-4, MIF2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34742

Provider

Invitrogen Antibodies

Product name

CENPC Recombinant Rabbit Monoclonal Antibody (JG36-15)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: K562, Daudi, SH-SY-5Y, mouse spleen tissue, rat heart tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JG36-15

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CENPC in K562 cell lysate. Samples were incubated with CENPC monoclonal antibody (Product # MA5-34742), at a dilution of 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of CENPC in SH-SY-5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with CENPC monoclonal antibody (Product # MA5-34742), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CENPC in paraffin-embedded rat heart tissue. Samples were heat mediated antigen retrieval with sodium citrate buffer (pH6, 20 min), incubated with CENPC monoclonal antibody (Product # MA5-34742), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CENPC in paraffin-embedded mouse spleen tissue. Samples were heat mediated antigen retrieval with sodium citrate buffer (pH6, 20 min), incubated with CENPC monoclonal antibody (Product # MA5-34742), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of CENPC in K562 cells (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple). Samples were incubated with CENPC monoclonal antibody (Product # MA5-34742) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.