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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-104398 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AOC3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of total AOC3.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Endothelial cell-derived SSAO can increase MLC(20) phosphorylation in VSMCs.
Zhang Y, Zhang X, Cao Z, Huang Y, Zheng Y, Yang X
Open life sciences 2021;16(1):1141-1150
Open life sciences 2021;16(1):1141-1150
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AOC3 in A549 cell lysate (left lane: treated with the antigen-specific peptide). Samples were incubated with AOC3 polyclonal antibody (Product # PA5-104398).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded AOC3 in human lymph tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with AOC3 polyclonal antibody (Product # PA5-104398) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Detection of AOC3 overexpression in RIMECs by adenovirus. (a) AOC3 mRNA expression in RIMECs as determined by qRT-PCR. GAPDH was used as an internal control. (b) SSAO protein expression in RIMECs was determined by Western-Blot. GAPDH was used as an internal control (* P < 0.05, ** P < 0.01, and *** P < 0.001).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 SSAO can regulate LPS-induced iNOS expression in RIMECs. (a) SSAO and iNOS activity in RIMECs culture medium were determined using Fluoro SSAO(tm) Fluorescent SSAO Detection kit and PathScan(r) Total iNOS Sandwich ELISA Kit. (b) SSAO and iNOS protein expression in RIMECs, as determined by the western blotting. GAPDH was used as an internal control (* P < 0.05, ** P < 0.01, and *** P < 0.001).
