HPA000275

Etle, etoile, SALP, SLM-2, SLM2, T-STAR

Eligible for validation within the Antibodypedia Validation Initiative

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Validation data

Product number: HPA000275
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA000275, RRID:AB_611575
Product name: Anti-KHDRBS3
Provider product page: Atlas Antibodies - HPA000275
Antibody type: Polyclonal
Description: Affinity purified using the PrEST antigen as affinity ligand
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: ADVPVVRGKPTLRTRGVPAPAITRGRGGVTARPVG VVVPRGTPTPRGVLSTRGPVSRGRGLLTPRARGVP PTGYRPPPPPPTQETYGEYDYDDGYGTAYDEQSYD S
Isotype: IgG
Vial size: 100 µl
Storage: Store at +4¡C for short term storage. Long time storage is recommended at -20¡C.

Provider	Type	Product Number
- No reagents -

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neurons.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human kidney shows moderate to strong nuclear positivity in cells in tubules.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human placenta shows no positivity in trophoblastic cells as expected.
Sample type: HUMAN

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Immunohistochemistry analysis in human testis and placenta tissues using HPA000275 antibody. Corresponding KHDRBS3 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.