PA5-54079

antibody from Invitrogen Antibodies

Targeting: DAZL DAZH, DAZL1, DAZLA, MGC26406, SPGYLA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-54079

Provider

Invitrogen Antibodies

Product name

DAZL Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: HEATPPSGNG PQKKSVDRSI QTVVSCLFNP ENRLRNSVVT QDDYFKDKRV HHFRRSRAML K

Concentration

0.4 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DAZL in control (vector only transfected HEK293T lysate) and DAZL over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a DAZL Polyclonal Antibody (Product # PA5-54079).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-DAZL Polyclonal Antibody (Product # PA5-54079) and a 35 kDa band corresponding to DAZL was observed only in Mouse Testis, Rat Testis and absent in Mouse Liver and Mouse Heart which are reported to be negative for DAZL expression. Tissue extracts (30 µg lysate) of Mouse Testis (Lane 1), Rat Testis (Lane 2), Mouse Liver (Lane 3) and Mouse Heart (Lane 4) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of DAZL in human cell line A-431 shows positivity in cytoplasm. Samples were probed using a DAZL Polyclonal Antibody (Product # PA5-54079).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of DAZL in human testis tissue shows strong cytoplasmic and nuclear positivity in cells of seminiferous ducts. Samples were probed using a DAZL Polyclonal Antibody (Product # PA5-54079).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of DAZL in human testis and endometrium tissues using DAZL Polyclonal Antibody (Product # PA5-54079). Corresponding DAZL RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of DAZL in human endometrium using DAZL Polyclonal Antibody (Product # PA5-54079) shows low expression as expected.