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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP2-61937 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal DAZL Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DAZL Antibody ([DAZL3/11A]) - Azide and BSA Free [NBP2-61937] - Clone 3/11A used to detect DAZL in ovarian and testicular tissue by Western Blot. Western analysis of 1st and 2nd trimester ovaries and testes. In both ovarian (A) and testicular (B) samples VASA (76 kDa) was not detectable in the 1st trimester samples, but was present in those from the 2nd trimester. DAZL (33 kDa) was low/undetectable in 1st trimester ovaries (C), whereas it was detectable in ovarian samples from 2nd trimester and testicular samples from both 1st and 2nd trimester (D). OCT4 (42 kDa) was present in both ovaries (E) and testes (F) during both the 1st and 2nd trimester. The loading control in all cases was beta-tubulin (51 kDa).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: DAZL Antibody ([DAZL3/11A]) - Azide and BSA Free [NBP2-61937] - Clone 3/11A used to detect DAZL expression in rat testis by IHC-P. Immunohistochemistry showing localization of germ cells and 5mC during mid to late gestation using DAZL as a cytoplasmic marker.
