PA5-88080

antibody from Invitrogen Antibodies

Targeting: DAZL DAZH, DAZL1, DAZLA, MGC26406, SPGYLA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-88080

Provider

Invitrogen Antibodies

Product name

DAZL Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: VYNYQMPPQW PVGEQRSYVV PPAYSAVNYH CNEVDPGAEV VPNECSVHEA TPPSGNGPQK KSVDRSIQTV VSCLFNPENR LRNSVVTQDD YFKDKRVHHF RRSRAMLKSV; Positive Samples: MCF7, SH-SY5Y, HL-60, SGC-7901, Mouse brain, Mouse stomach; Cellular Location: Cytoplasm, Nucleus

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.99 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of DAZL in extracts of various cell lines using DAZL Polyclonal Antibody (Product # PA5-88080) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using DAZL Polyclonal antibody (Product # PA5-88080) at dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of DAZL was performed in HeLa cells using DAZL Polyclonal Antibody (Product # PA5-88080).