16686-1-AP

antibody from Proteintech Group

Targeting: CKAP4 CLIMP-63, ERGIC-63, P63

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

16686-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#16686-1-AP, RRID:AB_2276275

Product name

CKAP4 antibody

Antibody type

Polyclonal

Description

CKAP4 antibody (Cat. #16686-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

SSR1 and CKAP4 as potential biomarkers for intervertebral disc degeneration based on integrated bioinformatics analysis.

Guo D, Zeng M, Yu M, Shang J, Lin J, Liu L, Yang K, Cao Z
JOR spine 2024 Mar;7(1):e1309

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Combinatorial selective ER-phagy remodels the ER during neurogenesis.

Hoyer MJ, Capitanio C, Smith IR, Paoli JC, Bieber A, Jiang Y, Paulo JA, Gonzalez-Lozano MA, Baumeister W, Wilfling F, Schulman BA, Harper JW
Nature cell biology 2024 Mar;26(3):378-392

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ATG14 targets lipid droplets and acts as an autophagic receptor for syntaxin18-regulated lipid droplet turnover.

Yuan Z, Cai K, Li J, Chen R, Zhang F, Tan X, Jiu Y, Chang H, Hu B, Zhang W, Ding B
Nature communications 2024 Jan 20;15(1):631

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Super-resolution microscopy reveals focal organization of ER-associated Y-complexes in mitosis.

Gandhimathi R, Pinotsi D, Köhler M, Mansfeld J, Ashiono C, Kleele T, Pawar S, Kutay U
EMBO reports 2023 Sep 6;24(9):e56766

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RNF26 binds perinuclear vimentin filaments to integrate ER and endolysosomal responses to proteotoxic stress.

Cremer T, Voortman LM, Bos E, Jongsma ML, Ter Haar LR, Akkermans JJ, Talavera Ormeño CM, Wijdeven RH, de Vries J, Kim RQ, Janssen GM, van Veelen PA, Koning RI, Neefjes J, Berlin I
The EMBO journal 2023 Sep 18;42(18):e111252

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Intrinsically disordered region-mediated condensation of IFN-inducible SCOTIN/SHISA-5 inhibits ER-to-Golgi vesicle transport.

Kim N, Kim TH, Kim C, Lee JE, Kang MG, Shin S, Jung M, Kim JS, Mun JY, Rhee HW, Park SY, Shin Y, Yoo JY
Developmental cell 2023 Oct 9;58(19):1950-1966.e8

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PACS-2 deficiency aggravates tubular injury in diabetic kidney disease by inhibiting ER-phagy.

Yang J, Li L, Li C, Chen W, Liu Y, Luo S, Zhao C, Han Y, Yang M, Zhao H, Jiang N, Xi Y, Tang C, Cai J, Xiao L, Liu H, Sun L
Cell death & disease 2023 Oct 4;14(10):649

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A regulatory circuit comprising the CBP and SIRT7 regulates FAM134B-mediated ER-phagy.

Wang X, Jiang X, Li B, Zheng J, Guo J, Gao L, Du M, Weng X, Li L, Chen S, Zhang J, Fang L, Liu T, Wang L, Liu W, Neculai D, Sun Q
The Journal of cell biology 2023 May 1;222(5)

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Deficiency of Perry syndrome-associated p150(Glued) in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities.

Yu J, Yang X, Zheng J, Sgobio C, Sun L, Cai H
NPJ Parkinson's disease 2023 Mar 7;9(1):35

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Heteromeric clusters of ubiquitinated ER-shaping proteins drive ER-phagy.

Foronda H, Fu Y, Covarrubias-Pinto A, Bocker HT, González A, Seemann E, Franzka P, Bock A, Bhaskara RM, Liebmann L, Hoffmann ME, Katona I, Koch N, Weis J, Kurth I, Gleeson JG, Reggiori F, Hummer G, Kessels MM, Qualmann B, Mari M, Dikić I, Hübner CA
Nature 2023 Jun;618(7964):402-410

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Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges.

Miyagi T, Ueda K, Sugimoto M, Yagi T, Ito D, Yamazaki R, Narumi S, Hayamizu Y, Uji-I H, Kuroda M, Kanekura K
iScience 2023 Jun 16;26(6):106957

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Palmitoylation couples insulin hypersecretion with β cell failure in diabetes.

Dong G, Adak S, Spyropoulos G, Zhang Q, Feng C, Yin L, Speck SL, Shyr Z, Morikawa S, Kitamura RA, Kathayat RS, Dickinson BC, Ng XW, Piston DW, Urano F, Remedi MS, Wei X, Semenkovich CF
Cell metabolism 2023 Feb 7;35(2):332-344.e7

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Coronavirus subverts ER-phagy by hijacking FAM134B and ATL3 into p62 condensates to facilitate viral replication.

Tan X, Cai K, Li J, Yuan Z, Chen R, Xiao H, Xu C, Hu B, Qin Y, Ding B
Cell reports 2023 Apr 25;42(4):112286

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Malondialdehyde-Acetaldehyde Extracellular Matrix Protein Adducts Attenuate Unfolded Protein Response During Alcohol and Smoking-Induced Pancreatitis.

Bhatia R, Thompson CM, Clement EJ, Ganguly K, Cox JL, Rauth S, Siddiqui JA, Mashiana SS, Jain M, Wyatt TA, Mashiana HS, Singh S, Woods NT, Kharbanda KK, Batra SK, Kumar S
Gastroenterology 2022 Oct;163(4):1064-1078.e10

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The mammalian rhomboid protein RHBDL4 protects against endoplasmic reticulum stress by regulating the morphology and distribution of ER sheets.

Lastun VL, Levet C, Freeman M
The Journal of biological chemistry 2022 Jun;298(6):101935

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Proteomic Mapping and Targeting of Mitotic Pericentriolar Material in Tumors Bearing Centrosome Amplification.

Xie B, Pu Y, Yang F, Chen W, Yue W, Ma J, Zhang N, Jiang Y, Wu J, Lin Y, Liang X, Wang C, Zou P, Li M
Cancer research 2022 Jul 18;82(14):2576-2592

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Targeting FAM134B-mediated reticulophagy activates sorafenib-induced ferroptosis in hepatocellular carcinoma.

Liu Z, Ma C, Wang Q, Yang H, Lu Z, Bi T, Xu Z, Li T, Zhang L, Zhang Y, Liu J, Wei X, Li J
Biochemical and biophysical research communications 2022 Jan 22;589:247-253

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RNA-Binding Profiles of CKAP4 as an RNA-Binding Protein in Myocardial Tissues.

Zhu H, Zhang Y, Zhang C, Xie Z
Frontiers in cardiovascular medicine 2021;8:773573

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ATF4 links ER stress with reticulophagy in glioblastoma cells.

Zielke S, Kardo S, Zein L, Mari M, Covarrubias-Pinto A, Kinzler MN, Meyer N, Stolz A, Fulda S, Reggiori F, Kögel D, van Wijk S
Autophagy 2021 Sep;17(9):2432-2448

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N-glycan processing selects ERAD-resistant misfolded proteins for ER-to-lysosome-associated degradation.

Fregno I, Fasana E, Soldà T, Galli C, Molinari M
The EMBO journal 2021 Aug 2;40(15):e107240

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Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.

van de Weijer ML, Krshnan L, Liberatori S, Guerrero EN, Robson-Tull J, Hahn L, Lebbink RJ, Wiertz EJHJ, Fischer R, Ebner D, Carvalho P
Molecular cell 2020 Sep 3;79(5):768-781.e7

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MiT/TFE factors control ER-phagy via transcriptional regulation of FAM134B.

Cinque L, De Leonibus C, Iavazzo M, Krahmer N, Intartaglia D, Salierno FG, De Cegli R, Di Malta C, Svelto M, Lanzara C, Maddaluno M, Wanderlingh LG, Huebner AK, Cesana M, Bonn F, Polishchuk E, Hübner CA, Conte I, Dikic I, Mann M, Ballabio A, Sacco F, Grumati P, Settembre C
The EMBO journal 2020 Sep 1;39(17):e105696

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Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.

van de Weijer ML, Krshnan L, Liberatori S, Guerrero EN, Robson-Tull J, Hahn L, Lebbink RJ, Wiertz EJHJ, Fischer R, Ebner D, Carvalho P
Molecular cell 2020 Oct 15;80(2):374-375

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Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation.

Yamanaka T, Nishiyama R, Shimogori T, Nukina N
Scientific reports 2020 May 6;10(1):7610

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The RBP1-CKAP4 axis activates oncogenic autophagy and promotes cancer progression in oral squamous cell carcinoma.

Gao L, Wang Q, Ren W, Zheng J, Li S, Dou Z, Kong X, Liang X, Zhi K
Cell death & disease 2020 Jun 25;11(6):488

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Rab18 regulates focal adhesion dynamics by interacting with kinectin-1 at the endoplasmic reticulum.

Guadagno NA, Margiotta A, Bjørnestad SA, Haugen LH, Kjos I, Xu X, Hu X, Bakke O, Margadant F, Progida C
The Journal of cell biology 2020 Jul 6;219(7)

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CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy.

Nthiga TM, Kumar Shrestha B, Sjøttem E, Bruun JA, Bowitz Larsen K, Bhujabal Z, Lamark T, Johansen T
The EMBO journal 2020 Aug 3;39(15):e103649

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BPIFB3 Regulates Endoplasmic Reticulum Morphology To Facilitate Flavivirus Replication.

Evans AS, Lennemann NJ, Coyne CB
Journal of virology 2020 Apr 16;94(9)

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Significant Down-Regulation of Urea Cycle Generates Clinically Relevant Proteomic Signature in Hepatocellular Carcinoma Patients with Macrovascular Invasion.

Cao Y, Ding W, Zhang J, Gao Q, Yang H, Cao W, Wang Z, Fang L, Du R
Journal of proteome research 2019 May 3;18(5):2032-2044

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SENCR stabilizes vascular endothelial cell adherens junctions through interaction with CKAP4.

Lyu Q, Xu S, Lyu Y, Choi M, Christie CK, Slivano OJ, Rahman A, Jin ZG, Long X, Xu Y, Miano JM
Proceedings of the National Academy of Sciences of the United States of America 2019 Jan 8;116(2):546-555

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Excessive ER-phagy mediated by the autophagy receptor FAM134B results in ER stress, the unfolded protein response, and cell death in HeLa cells.

Liao Y, Duan B, Zhang Y, Zhang X, Xia B
The Journal of biological chemistry 2019 Dec 27;294(52):20009-20023

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MicroRNA-671-3p promotes proliferation and migration of glioma cells via targeting CKAP4.

Lu GF, You CY, Chen YS, Jiang H, Zheng X, Tang WW, Wang XY, Xu HY, Geng F
OncoTargets and therapy 2018;11:6217-6226

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ER-to-lysosome-associated degradation of proteasome-resistant ATZ polymers occurs via receptor-mediated vesicular transport.

Fregno I, Fasana E, Bergmann TJ, Raimondi A, Loi M, Soldà T, Galli C, D'Antuono R, Morone D, Danieli A, Paganetti P, van Anken E, Molinari M
The EMBO journal 2018 Sep 3;37(17)

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Full length RTN3 regulates turnover of tubular endoplasmic reticulum via selective autophagy.

Grumati P, Morozzi G, Hölper S, Mari M, Harwardt MI, Yan R, Müller S, Reggiori F, Heilemann M, Dikic I
eLife 2017 Jun 15;6

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Dengue and Zika viruses subvert reticulophagy by NS2B3-mediated cleavage of FAM134B.

Lennemann NJ, Coyne CB
Autophagy 2017 Feb;13(2):322-332

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Sequestosome 1/p62 Protein Is Associated with Autophagic Removal of Excess Hepatic Endoplasmic Reticulum in Mice.

Yang H, Ni HM, Guo F, Ding Y, Shi YH, Lahiri P, Fröhlich LF, Rülicke T, Smole C, Schmidt VC, Zatloukal K, Cui Y, Komatsu M, Fan J, Ding WX
The Journal of biological chemistry 2016 Sep 2;291(36):18663-74

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Regulation of endoplasmic reticulum turnover by selective autophagy.

Khaminets A, Heinrich T, Mari M, Grumati P, Huebner AK, Akutsu M, Liebmann L, Stolz A, Nietzsche S, Koch N, Mauthe M, Katona I, Qualmann B, Weis J, Reggiori F, Kurth I, Hübner CA, Dikic I
Nature 2015 Jun 18;522(7556):354-8

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The endogenous caspase-8 inhibitor c-FLIPL regulates ER morphology and crosstalk with mitochondria.

Marini ES, Giampietri C, Petrungaro S, Conti S, Filippini A, Scorrano L, Ziparo E
Cell death and differentiation 2015 Jul;22(7):1131-43

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 16686-1-AP(CKAP4 antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using CKAP4 antibody 16686-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human kidney using 16686-1-AP(CKAP4 antibody) at dilution of 1:200 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human kidney using 16686-1-AP(CKAP4 antibody) at dilution of 1:200 (under 40x lens)

Sample type

tissue