16686-1-AP

antibody from Proteintech Group

Targeting: CKAP4 CLIMP-63, ERGIC-63, P63

Provider product page for 16686-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [63]
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Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 16686-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#16686-1-AP, RRID:AB_2276275
Product name: CKAP4 antibody
Antibody type: Polyclonal
Description: CKAP4 antibody (Cat. #16686-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

CKAP4 protein structure - 16686-1-AP shown in red.

Submitted references Decoding the DKK1/CKAP4 signaling Axis: A novel mechanism driving Neointimal hyperplasia in arteriovenous fistulas.

Xu S, Liang L, Xu H, Wang Q, Wei K, Wang Z, Kong X
International immunopharmacology 2026 May 15;177:116520

Long noncoding RNA AC007637.1 inhibits tumorigenesis by regulating the Trim25/PCNA axis in colorectal cancer.

Wang X, Li J, Hao C, Cui K, Tian L, Zhao J, Li C, Feng Y, Yao S, Fei B, Mao X, Huang Z
Journal of advanced research 2026 Apr 6;

CKAP4 and PLOD2 as novel prognostic biomarkers in hepatocellular carcinoma: a proteomics-driven risk stratification model.

Lu Q, Cao Z, Xiong Y, Huang J, Zeng H, Chen Z, Shu Y, Tan Y, Long X, Liu X, Shu H
Frontiers in cell and developmental biology 2025;13:1577161

Endoplasmic reticulum-anchored nonstructural proteins drive human astrovirus replication organelle formation.

Bengert B, Mehri S, Holliday M, Lennemann NJ
PLoS pathogens 2025 Sep;21(9):e1013538

The lncRNA MIR4435-2HG Modulates Bladder Cancer Progression and Ferroptosis Through the IQGAP3/Ras/ERK/CREB Pathway.

Yang T, Guo L, Kong S, Xu Q, Zhang P, Zhan C, Wang X, Wang G
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2025 Oct 31;39(20):e71119

Identification and characterization of a ubiquitin E3 RING ligase of the Chlamydia-like bacterium Simkania negevensis.

Hörner EM, Lachmayer V, Hermanns T, Moldovan A, Hofmann K, Kozjak-Pavlovic V
PLoS pathogens 2025 Nov;21(11):e1013626

Rab GTPases are evolutionarily conserved signals mediating selective autophagy.

Zhao P, Tian R, Song D, Zhu Q, Ding X, Zhang J, Cao B, Zhang M, Xu Y, Fang J, Tan J, Yi C, Xia H, Liu W, Zou W, Sun Q
The Journal of cell biology 2025 May 5;224(5)

Parvimonas micra promotes oral squamous cell carcinoma metastasis through TmpC-CKAP4 axis.

Qi H, Gao H, Li M, Sun T, Gu X, Wei L, Zhi M, Li Z, Fu D, Liu Y, Wei Z, Dou Y, Feng Q
Nature communications 2025 Mar 7;16(1):2305

Endoplasmic reticulum-anchored nonstructural proteins drive human astrovirus replication organelle formation.

Bengert B, Mehri S, Holliday M, Lennemann NJ
bioRxiv : the preprint server for biology 2025 Jun 25;

PTPA localized in the Golgi apparatus plays an important role in osteoblast differentiation.

Zheng X, Weng Y, Satoh A, Tanai A, Ikegame M, Kimura K, Yoshitani N, Xie X, Okamura H
Biochemical and biophysical research communications 2025 Feb 8;748:151329

CKAP4 in Extracellular Vesicle-Derived From Podocyte Serves as a Non-Invasive Diagnostic Biomarker for Diabetic Nephropathy and Promotes Vascular Calcification.

Zhang H, Lou K, Qian L, Zhang M, Li J, Lv L, Lu F, He G, Wu C, Wu J, Jia M, Bai Y, Qu S, Liu M, Chu L, Wang Y, Ding D, Zen K, Yuan Y, Liang H
Journal of extracellular vesicles 2025 Dec;14(12):e70208

Unveiling key genes for intervertebral disc degeneration prediction and potential drug discovery.

Li TJ, Wang YS, Lan BB, Liu JW
Scientific reports 2025 Dec 11;16(1):1918

CKAP4 in hepatocellular carcinoma: competitive RETREG1/FAM134B binding, reticulophagy regulation, and cancer progression.

Mo J, Su C, Li P, Yang Z, Tao R, Liu Q, Yuan C, Xu L, Ge Q, Ning D, Liang H, Zhu H, Luo Y, Chen X, Chen J, Zhang B
Autophagy 2025 Apr;21(4):840-859

The assembly of RAB22A/TMEM33/RTN4 initiates a secretory ER-phagy pathway.

Zheng X, Fang D, Shan H, Xiao B, Wei D, Ouyang Y, Huo L, Zhang Z, Wu Y, Zhang R, Kang T, Gao Y
Cell discovery 2025 Apr 29;11(1):41

The COPII Transport Complex Participates in HPV16 Infection.

Day PM, Thompson CD, Weisberg AS, Schiller JT
Viruses 2025 Apr 25;17(5)

Identification of immune-related biomarkers for intracerebral hemorrhage diagnosis based on RNA sequencing and machine learning.

Bai C, Liu X, Wang F, Sun Y, Wang J, Liu J, Hao X, Zhou L, Yuan Y, Liu J
Frontiers in immunology 2024;15:1421942

LW-213, a derivative of wogonin, triggers reticulophagy-mediated cell death in NSCLC via lysosomal damage combined with NPC1 inhibition.

Wang H, Jiang Y, Zhu M, Li H, Chen H, Wang H, Zhang S, Guo Q, Hui H
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 Nov;134:155958

AMFR-mediated Flavivirus NS2A ubiquitination subverts ER-phagy to augment viral pathogenicity.

Zhang L, Wang H, Han C, Dong Q, Yan J, Guo W, Shan C, Zhao W, Chen P, Huang R, Wu Y, Chen Y, Qin Y, Chen M
Nature communications 2024 Nov 6;15(1):9578

Cancer cells sense solid stress to enhance metastasis by CKAP4 phase separation-mediated microtubule branching.

Sun X, Zhou Y, Sun S, Qiu S, Peng M, Gong H, Guo J, Wen C, Zhang Y, Xie Y, Li H, Liang L, Luo G, Wu W, Liu J, Tan W, Ye M
Cell discovery 2024 Nov 12;10(1):114

SSR1 and CKAP4 as potential biomarkers for intervertebral disc degeneration based on integrated bioinformatics analysis.

Guo D, Zeng M, Yu M, Shang J, Lin J, Liu L, Yang K, Cao Z
JOR spine 2024 Mar;7(1):e1309

Combinatorial selective ER-phagy remodels the ER during neurogenesis.

Hoyer MJ, Capitanio C, Smith IR, Paoli JC, Bieber A, Jiang Y, Paulo JA, Gonzalez-Lozano MA, Baumeister W, Wilfling F, Schulman BA, Harper JW
Nature cell biology 2024 Mar;26(3):378-392

The glycosylation deficiency of flavivirus NS1 attenuates virus replication through interfering with the formation of viral replication compartments.

Huang S, Shi PD, Fan XX, Yang Y, Qin CF, Zhao H, Shi L, Ci Y
Journal of biomedical science 2024 Jun 7;31(1):60

Multiscale photocatalytic proximity labeling reveals cell surface neighbors on and between cells.

Lin Z, Schaefer K, Lui I, Yao Z, Fossati A, Swaney DL, Palar A, Sali A, Wells JA
Science (New York, N.Y.) 2024 Jul 19;385(6706):eadl5763

ATG14 targets lipid droplets and acts as an autophagic receptor for syntaxin18-regulated lipid droplet turnover.

Yuan Z, Cai K, Li J, Chen R, Zhang F, Tan X, Jiu Y, Chang H, Hu B, Zhang W, Ding B
Nature communications 2024 Jan 20;15(1):631

Visualizing ER-phagy and ER architecture in vivo.

Sang Y, Li B, Su T, Zhan H, Xiong Y, Huang Z, Wang C, Cong X, Du M, Wu Y, Yu H, Yang X, Ding K, Wang X, Miao X, Gong W, Wang L, Zhao J, Zhou Y, Liu W, Hu X, Sun Q
The Journal of cell biology 2024 Dec 2;223(12)

Single-cell transcriptomics of vomeronasal neuroepithelium reveals a differential endoplasmic reticulum environment amongst neuronal subtypes.

Devakinandan GVS, Terasaki M, Dani A
eLife 2024 Dec 13;13

Topology surveillance of the lanosterol demethylase CYP51A1 by signal peptide peptidase.

Sergejevs N, Avci D, van de Weijer ML, Corey RA, Lemberg MK, Carvalho P
Journal of cell science 2024 Dec 1;137(23)

Sestrin2 drives ER-phagy in response to protein misfolding.

De Leonibus C, Maddaluno M, Ferriero R, Besio R, Cinque L, Lim PJ, Palma A, De Cegli R, Gagliotta S, Montefusco S, Iavazzo M, Rohrbach M, Giunta C, Polishchuk E, Medina DL, Di Bernardo D, Forlino A, Piccolo P, Settembre C
Developmental cell 2024 Aug 19;59(16):2035-2052.e10

Super-resolution microscopy reveals focal organization of ER-associated Y-complexes in mitosis.

Gandhimathi R, Pinotsi D, Köhler M, Mansfeld J, Ashiono C, Kleele T, Pawar S, Kutay U
EMBO reports 2023 Sep 6;24(9):e56766

RNF26 binds perinuclear vimentin filaments to integrate ER and endolysosomal responses to proteotoxic stress.

Cremer T, Voortman LM, Bos E, Jongsma ML, Ter Haar LR, Akkermans JJ, Talavera Ormeño CM, Wijdeven RH, de Vries J, Kim RQ, Janssen GM, van Veelen PA, Koning RI, Neefjes J, Berlin I
The EMBO journal 2023 Sep 18;42(18):e111252

Intrinsically disordered region-mediated condensation of IFN-inducible SCOTIN/SHISA-5 inhibits ER-to-Golgi vesicle transport.

Kim N, Kim TH, Kim C, Lee JE, Kang MG, Shin S, Jung M, Kim JS, Mun JY, Rhee HW, Park SY, Shin Y, Yoo JY
Developmental cell 2023 Oct 9;58(19):1950-1966.e8

PACS-2 deficiency aggravates tubular injury in diabetic kidney disease by inhibiting ER-phagy.

Yang J, Li L, Li C, Chen W, Liu Y, Luo S, Zhao C, Han Y, Yang M, Zhao H, Jiang N, Xi Y, Tang C, Cai J, Xiao L, Liu H, Sun L
Cell death & disease 2023 Oct 4;14(10):649

A regulatory circuit comprising the CBP and SIRT7 regulates FAM134B-mediated ER-phagy.

Wang X, Jiang X, Li B, Zheng J, Guo J, Gao L, Du M, Weng X, Li L, Chen S, Zhang J, Fang L, Liu T, Wang L, Liu W, Neculai D, Sun Q
The Journal of cell biology 2023 May 1;222(5)

Deficiency of Perry syndrome-associated p150(Glued) in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities.

Yu J, Yang X, Zheng J, Sgobio C, Sun L, Cai H
NPJ Parkinson's disease 2023 Mar 7;9(1):35

Heteromeric clusters of ubiquitinated ER-shaping proteins drive ER-phagy.

Foronda H, Fu Y, Covarrubias-Pinto A, Bocker HT, González A, Seemann E, Franzka P, Bock A, Bhaskara RM, Liebmann L, Hoffmann ME, Katona I, Koch N, Weis J, Kurth I, Gleeson JG, Reggiori F, Hummer G, Kessels MM, Qualmann B, Mari M, Dikić I, Hübner CA
Nature 2023 Jun;618(7964):402-410

Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges.

Miyagi T, Ueda K, Sugimoto M, Yagi T, Ito D, Yamazaki R, Narumi S, Hayamizu Y, Uji-I H, Kuroda M, Kanekura K
iScience 2023 Jun 16;26(6):106957

Palmitoylation couples insulin hypersecretion with β cell failure in diabetes.

Dong G, Adak S, Spyropoulos G, Zhang Q, Feng C, Yin L, Speck SL, Shyr Z, Morikawa S, Kitamura RA, Kathayat RS, Dickinson BC, Ng XW, Piston DW, Urano F, Remedi MS, Wei X, Semenkovich CF
Cell metabolism 2023 Feb 7;35(2):332-344.e7

Coronavirus subverts ER-phagy by hijacking FAM134B and ATL3 into p62 condensates to facilitate viral replication.

Tan X, Cai K, Li J, Yuan Z, Chen R, Xiao H, Xu C, Hu B, Qin Y, Ding B
Cell reports 2023 Apr 25;42(4):112286

Malondialdehyde-Acetaldehyde Extracellular Matrix Protein Adducts Attenuate Unfolded Protein Response During Alcohol and Smoking-Induced Pancreatitis.

Bhatia R, Thompson CM, Clement EJ, Ganguly K, Cox JL, Rauth S, Siddiqui JA, Mashiana SS, Jain M, Wyatt TA, Mashiana HS, Singh S, Woods NT, Kharbanda KK, Batra SK, Kumar S
Gastroenterology 2022 Oct;163(4):1064-1078.e10

The mammalian rhomboid protein RHBDL4 protects against endoplasmic reticulum stress by regulating the morphology and distribution of ER sheets.

Lastun VL, Levet C, Freeman M
The Journal of biological chemistry 2022 Jun;298(6):101935

Proteomic Mapping and Targeting of Mitotic Pericentriolar Material in Tumors Bearing Centrosome Amplification.

Xie B, Pu Y, Yang F, Chen W, Yue W, Ma J, Zhang N, Jiang Y, Wu J, Lin Y, Liang X, Wang C, Zou P, Li M
Cancer research 2022 Jul 18;82(14):2576-2592

Targeting FAM134B-mediated reticulophagy activates sorafenib-induced ferroptosis in hepatocellular carcinoma.

Liu Z, Ma C, Wang Q, Yang H, Lu Z, Bi T, Xu Z, Li T, Zhang L, Zhang Y, Liu J, Wei X, Li J
Biochemical and biophysical research communications 2022 Jan 22;589:247-253

RNA-Binding Profiles of CKAP4 as an RNA-Binding Protein in Myocardial Tissues.

Zhu H, Zhang Y, Zhang C, Xie Z
Frontiers in cardiovascular medicine 2021;8:773573

ATF4 links ER stress with reticulophagy in glioblastoma cells.

Zielke S, Kardo S, Zein L, Mari M, Covarrubias-Pinto A, Kinzler MN, Meyer N, Stolz A, Fulda S, Reggiori F, Kögel D, van Wijk S
Autophagy 2021 Sep;17(9):2432-2448

N-glycan processing selects ERAD-resistant misfolded proteins for ER-to-lysosome-associated degradation.

Fregno I, Fasana E, Soldà T, Galli C, Molinari M
The EMBO journal 2021 Aug 2;40(15):e107240

Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.

van de Weijer ML, Krshnan L, Liberatori S, Guerrero EN, Robson-Tull J, Hahn L, Lebbink RJ, Wiertz EJHJ, Fischer R, Ebner D, Carvalho P
Molecular cell 2020 Sep 3;79(5):768-781.e7

MiT/TFE factors control ER-phagy via transcriptional regulation of FAM134B.

Cinque L, De Leonibus C, Iavazzo M, Krahmer N, Intartaglia D, Salierno FG, De Cegli R, Di Malta C, Svelto M, Lanzara C, Maddaluno M, Wanderlingh LG, Huebner AK, Cesana M, Bonn F, Polishchuk E, Hübner CA, Conte I, Dikic I, Mann M, Ballabio A, Sacco F, Grumati P, Settembre C
The EMBO journal 2020 Sep 1;39(17):e105696

Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.

van de Weijer ML, Krshnan L, Liberatori S, Guerrero EN, Robson-Tull J, Hahn L, Lebbink RJ, Wiertz EJHJ, Fischer R, Ebner D, Carvalho P
Molecular cell 2020 Oct 15;80(2):374-375

Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation.

Yamanaka T, Nishiyama R, Shimogori T, Nukina N
Scientific reports 2020 May 6;10(1):7610

The RBP1-CKAP4 axis activates oncogenic autophagy and promotes cancer progression in oral squamous cell carcinoma.

Gao L, Wang Q, Ren W, Zheng J, Li S, Dou Z, Kong X, Liang X, Zhi K
Cell death & disease 2020 Jun 25;11(6):488

Rab18 regulates focal adhesion dynamics by interacting with kinectin-1 at the endoplasmic reticulum.

Guadagno NA, Margiotta A, Bjørnestad SA, Haugen LH, Kjos I, Xu X, Hu X, Bakke O, Margadant F, Progida C
The Journal of cell biology 2020 Jul 6;219(7)

CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy.

Nthiga TM, Kumar Shrestha B, Sjøttem E, Bruun JA, Bowitz Larsen K, Bhujabal Z, Lamark T, Johansen T
The EMBO journal 2020 Aug 3;39(15):e103649

BPIFB3 Regulates Endoplasmic Reticulum Morphology To Facilitate Flavivirus Replication.

Evans AS, Lennemann NJ, Coyne CB
Journal of virology 2020 Apr 16;94(9)

Significant Down-Regulation of Urea Cycle Generates Clinically Relevant Proteomic Signature in Hepatocellular Carcinoma Patients with Macrovascular Invasion.

Cao Y, Ding W, Zhang J, Gao Q, Yang H, Cao W, Wang Z, Fang L, Du R
Journal of proteome research 2019 May 3;18(5):2032-2044

SENCR stabilizes vascular endothelial cell adherens junctions through interaction with CKAP4.

Lyu Q, Xu S, Lyu Y, Choi M, Christie CK, Slivano OJ, Rahman A, Jin ZG, Long X, Xu Y, Miano JM
Proceedings of the National Academy of Sciences of the United States of America 2019 Jan 8;116(2):546-555

Excessive ER-phagy mediated by the autophagy receptor FAM134B results in ER stress, the unfolded protein response, and cell death in HeLa cells.

Liao Y, Duan B, Zhang Y, Zhang X, Xia B
The Journal of biological chemistry 2019 Dec 27;294(52):20009-20023

MicroRNA-671-3p promotes proliferation and migration of glioma cells via targeting CKAP4.

Lu GF, You CY, Chen YS, Jiang H, Zheng X, Tang WW, Wang XY, Xu HY, Geng F
OncoTargets and therapy 2018;11:6217-6226

ER-to-lysosome-associated degradation of proteasome-resistant ATZ polymers occurs via receptor-mediated vesicular transport.

Fregno I, Fasana E, Bergmann TJ, Raimondi A, Loi M, Soldà T, Galli C, D'Antuono R, Morone D, Danieli A, Paganetti P, van Anken E, Molinari M
The EMBO journal 2018 Sep 3;37(17)

Full length RTN3 regulates turnover of tubular endoplasmic reticulum via selective autophagy.

Grumati P, Morozzi G, Hölper S, Mari M, Harwardt MIE, Yan R, Müller S, Reggiori F, Heilemann M, Dikic I
eLife 2017 Jun 15;6

Dengue and Zika viruses subvert reticulophagy by NS2B3-mediated cleavage of FAM134B.

Lennemann NJ, Coyne CB
Autophagy 2017 Feb;13(2):322-332

Sequestosome 1/p62 Protein Is Associated with Autophagic Removal of Excess Hepatic Endoplasmic Reticulum in Mice.

Yang H, Ni HM, Guo F, Ding Y, Shi YH, Lahiri P, Fröhlich LF, Rülicke T, Smole C, Schmidt VC, Zatloukal K, Cui Y, Komatsu M, Fan J, Ding WX
The Journal of biological chemistry 2016 Sep 2;291(36):18663-74

Regulation of endoplasmic reticulum turnover by selective autophagy.

Khaminets A, Heinrich T, Mari M, Grumati P, Huebner AK, Akutsu M, Liebmann L, Stolz A, Nietzsche S, Koch N, Mauthe M, Katona I, Qualmann B, Weis J, Reggiori F, Kurth I, Hübner CA, Dikic I
Nature 2015 Jun 18;522(7556):354-8

The endogenous caspase-8 inhibitor c-FLIPL regulates ER morphology and crosstalk with mitochondria.

Marini ES, Giampietri C, Petrungaro S, Conti S, Filippini A, Scorrano L, Ziparo E
Cell death and differentiation 2015 Jul;22(7):1131-43

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HepG2 cells were subjected to SDS PAGE followed by western blot with 16686-1-AP(CKAP4 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using CKAP4 antibody 16686-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human kidney using 16686-1-AP(CKAP4 antibody) at dilution of 1:200 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human kidney using 16686-1-AP(CKAP4 antibody) at dilution of 1:200 (under 40x lens)
Sample type: tissue