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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-31639 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CKAP4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, HepG2, HCT116, MDA-MB-231, aortic smooth muscle. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.19 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CKAP4 Polyclonal Antibody (Product # PA5-31639). Sample (30 µg of whole cell lysate). Lane A: Hela . 7.5% SDS PAGE. CKAP4 Polyclonal Antibody (Product # PA5-31639) diluted at 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CKAP4 in methanol-fixed HeLa cells using a CKAP4 polyclonal antibody (Product # PA5-31639) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CKAP4 Polyclonal Antibody detects CKAP4 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: CKAP4 stained by CKAP4 Polyclonal Antibody (Product # PA5-31639) diluted at 1:1,000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:1,000. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CKAP4 Polyclonal Antibody detects CKAP4 protein at cytoplasm and membrane in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. CKAP4 Polyclonal Antibody (Product # PA5-31639) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using CKAP4 (Product # PA5-31639) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
