Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
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Validation data
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- Product number
- PA5-45794 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPL36AL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: FRKKAKTTKK IVLRLECVEP NCRSKRMLAI KRCKHFELGG DKKRKGQVIQ Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 92%; Zebrafish: 100%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2) and NIH/3T3 (Lane 3). The blot was probed with Anti-RPL36AL Polyclonal Antibody (Product # PA5-45794, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 15 kDa band corresponding to RPL36AL was observed across the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human adult placenta cells using an anti-RPL36AL polyclonal antibody (Product # PA5-45794).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Jurkat cells using an anti-RPL36AL polyclonal antibody (Product # PA5-45794).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of RPL36AL was achieved by transfecting HeLa cells with RPL36AL specific siRNAs (Silencer® select Product # s12228, s12229). Western blot analysis (Fig. a) was performed using whole cell extracts from the RPL36AL knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with RPL36AL Polyclonal Antibody (Product # PA5-45794, 1µg/ml) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to RPL36AL.