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Immunocytochemistry
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-48287 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- B7-H6 Chimeric Recombinant Rabbit Monoclonal Antibody (17B1.3)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Specificity: mAb 17B1.3 specifically recognises B7-H6. 17B1.3 reacted with human tumour cell lines constitutively expressing B7-H6 such as HeLa cells. Fab 17B1.3 does not interfere with the binding of B7-H6 to NKp30, but nevertheless blocks NK cell activation.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 17B1.3
- Vial size
- 200 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of B7-H6 in K562 cells using B7-H6 Chimeric Monoclonal Antibody (Product # MA5-48287). Paraformaldehyde-fixed cells were immobilized on cover slips and stained with B7-H6 Chimeric Monoclonal Antibody at 10 µg/mL for 1h followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom B7-H6 Chimeric Monoclonal Antibody, DAPI, merged channels and an isotype control. The isotype control was a Mouse IgG1 Isotype Control Antibody (Product # MA5-47823) followed by staining with Alexa Fluor 488 secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of B7-H6 in HeLa cells using B7-H6 Chimeric Monoclonal Antibody (Product # MA5-48287). Cells were fixed with paraformaldehyde and stained with Rabbit IgG Isotype Control (Product # MA5-47825) (black line) or B7-H6 Chimeric Monoclonal Antibody (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AF488 antibody at a dilution of 1:1,000 and cells analyzed using a flow-cytometer.
