LS-C377707

antibody from LSBio

Targeting: PDCD4 H731

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C377707

Provider

LSBio

Product name

PDCD4 Antibody (aa1-260) LS-C377707

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating PDCD4 in Hela whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of PDCD4 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: PDCD4 Antibody (4µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating PDCD4 in Hela whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of PDCD4 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: PDCD4 Antibody (4µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating PDCD4 in Hela whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of PDCD4 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: PDCD4 Antibody (4µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)