PAB10308

antibody from Abnova Corporation

Targeting: PDCD4 H731

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB10308

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB10308, RRID:AB_1676231

Product name

PDCD4 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of PDCD4.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Epidermal expression of the translation inhibitor programmed cell death 4 suppresses tumorigenesis.

Jansen AP, Camalier CE, Colburn NH
Cancer research 2005 Jul 15;65(14):6034-41

---

Akt phosphorylates and regulates Pdcd4 tumor suppressor protein.

Palamarchuk A, Efanov A, Maximov V, Aqeilan RI, Croce CM, Pekarsky Y
Cancer research 2005 Dec 15;65(24):11282-6

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using PDCD4 polyclonal antibody (Cat # PAB10308) shows detection of a band ~52 kDa in size corresponding to PDCD4 (arrowhead).Lane 1 contains recombinant PDCD4.Lane 2 contains 293 HEK cells treated with TPA and MG132.The PDCD4 polyclonal antibody was used at a 1 : 5,000 dilution.Personal Communication. M Young & A Jansen, NCI, Bethesda, MD.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

PDCD4 polyclonal antibody (Cat # PAB10308) was used at a 1 : 100 dilution to detect PDCD4 by immunohistochemistry on mouse colon tissue.Tissue was fixed in 4% paraformaldehyde and paraffin embedded.Tissue sections were deparaffinized and treated by trypsinization before staining.Personal Communication. M Young, NCI, Bethesda, MD.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)