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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- MP01105 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Pdcd4 phospho S457 Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Mouse Monoclonal antibody for PDCD4 detection. Tested positive for ELISA,Immunohistochemistry,Western Blot, in human, mouse, rat, xenopus
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Vial size
- 100ug
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of PDCD4 using anti-PDCD4 antibody (MP01105).PDCD4 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PDCD4 Antibody (MP01105) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
- Additional image
