933501

antibody from BioLegend

Targeting: PDCD4 H731

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

933501

Provider

BioLegend

Product name

Purified anti-PDCD4

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Rat

Conjugate

Unconjugated

Antibody clone number

W18083A

Vial size

25 µg

Concentration

0.50 mg/mL

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of purified anti-PDCD4 Antibody (clone W18083A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.

Conjugate

Unconjugated