HPA001032

PDCD4 antibody from Atlas Antibodies

H731

Eligible for validation within the Antibodypedia Validation Initiative

Western blot

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

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Antibody Data

Product number

HPA001032

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA001032, RRID:AB_1079586

Product name

Anti-PDCD4

Provider product page

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

VMSTTDVEKSFDKLLKDLPELALDTPRAPQLVGQF
IARAVGDGILCNTYIDSYKGTVDCVQARAALDKAT
VLLSMSKGGKRKDSVWGSGGGQQSVNHLVKE


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

MicroRNA Profiles in Familial and Sporadic Medullary Thyroid Carcinoma: Preliminary Relationships with Status and Outcome
Mian C, Pennelli G, Fassan M, Balistreri M, Barollo S, Cavedon E, Galuppini F, Pizzi M, Vianello F, Pelizzo M, Girelli M, Rugge M, Opocher G
Thyroid 2012 September;22(9):890-896

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Programmed cell death 4 nuclear loss and miR-21 or activated Akt overexpression in esophageal squamous cell carcinogenesis
Fassan M, Realdon S, Pizzi M, Balistreri M, Battaglia G, Zaninotto G, Ancona E, Rugge M
Diseases of the Esophagus 2012 April;25(3):263-268

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PDCD4 nuclear loss inversely correlates with miR-21 levels in colon carcinogenesis
Fassan M, Pizzi M, Giacomelli L, Mescoli C, Ludwig K, Pucciarelli S, Rugge M
Virchows Archiv 2011 April;458(4):413-419

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Programmed cell death 4 (PDCD4) expression during multistep Barrett's carcinogenesis
Fassan M, Pizzi M, Battaglia G, Giacomelli L, Parente P, Bocus P, Ancona E, Rugge M
Journal of Clinical Pathology 2010 August;63(8):692-696

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MicroRNA expression profiling of human metastatic cancers identifies cancer gene targets
Baffa R, Fassan M, Volinia S, O'Hara B, Liu C, Palazzo J, Gardiman M, Rugge M, Gomella L, Croce C, Rosenberg A
The Journal of Pathology 2009 October;219(2):214-221

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Tissue Profiling of the Mammalian Central Nervous System Using Human Antibody-based Proteomics
Mulder J, Bjorling E, Jonasson K, Wernerus H, Hober S, Hokfelt T, Uhlen M
Molecular & Cellular Proteomics 2009 July;8(7):1612-1622

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From Gene Expression Analysis to Tissue Microarrays: A Rational Approach to Identify Therapeutic and Diagnostic Targets in Lymphoid Malignancies
Ek S
Molecular & Cellular Proteomics 2006 March;5(6):1072-1081

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line Daudi.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human thyroid gland shows strong nuclear positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human thyroid gland shows strong nuclear positivity in glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human breast shows strong nuclear positivity in glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skin shows strong nuclear positivity in epidermal cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human small intestine shows strong nuclear positivity in glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows no positivity in myocytes as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human breast and skeletal muscle tissues using HPA001032 antibody. Corresponding PDCD4 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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