Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunoprecipitation [1]
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Validation data
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- Product number
- NB100-58785 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-58785, RRID:AB_877712
- Product name
- Rabbit Polyclonal PDCD4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Coupled activation and degradation of eEF2K regulates protein synthesis in response to genotoxic stress.
Kruiswijk F, Yuniati L, Magliozzi R, Low TY, Lim R, Bolder R, Mohammed S, Proud CG, Heck AJ, Pagano M, Guardavaccaro D
Science signaling 2012 Jun 5;5(227):ra40
Science signaling 2012 Jun 5;5(227):ra40
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunoprecipitation: PDCD4 Antibody [NB100-58785] - Detection of Human PDCD4 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PDCD4 antibody NB100-58785 used for IP at 3 mcg/mg lysate. PDCD4 was also immunoprecipitated by other rabbit anti-PDCD4 antibodies and NB100-58786, which recognize downstream epitopes. Detection: Chemiluminescence with an exposure time of 30 seconds.