AP02805PU-N

antibody from Acris Antibodies GmbH

Targeting: PDCD4 H731

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP02805PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP02805PU-N, RRID:AB_1622124

Product name

anti PDCD4

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding amino acids near the carboxyl terminus of Human Pdcd4 protein.

Reactivity

Human, Mouse, Rat, Xenopus

Host

Rabbit

Vial size

0.1 mg

Concentration

1.06 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using AP02805PU-N Pdcd4 antibody shows detection of a band ~52 kDa in size corresponding to Pdcd4 (arrowhead). Lane 1 contains recombinant Pdcd4. Lane 2 contains 293 HEK cells treated with TPA and MG132. The anti-Pdcd4 antibody was used at a 1/5,000 dilution. Personal Communication. M Young & A Jansen, NCI, Bethesda, MD.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

AP02805PU-N Pdcd4 antibody stainng Mouse colon tissue at 1/100 dilution. Tissue was fixed in 4% Paraformaldehyde and Paraffin Embedded. Tissue sections were deparaffinized and treated by trypsinization before staining. Personal Communication. M Young, NCI, Bethesda, MD.