AM08441PU-N

antibody from Acris Antibodies GmbH

Targeting: PDCD4 H731

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM08441PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM08441PU-N, RRID:AB_2035836

Product name

anti PDCD4 pSer457

Antibody type

Monoclonal

Antigen

Synthetic peptide corresponding to residues surrounding Ser457 of the Human Pdcd4 protein.

Reactivity

Human, Mouse, Rat, Xenopus

Host

Mouse

Isotype

IgG

Antibody clone number

9G6

Vial size

0.1 mg

Concentration

1.0 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot Protein A purified Mouse Monoclonal anti-Pdcd4 pS457 antibody shows detection of phosphorylated Pdcd4 (indicated by arrowhead at ~62 kDa) in NIH-3T3 cells after 5 min treatment with 30 ng/mL PDGF (lane 2). No reactivity is seen for unstimulated (non-phosphorylated) NIH 3T3 cells (lane 1). The membrane was probed with the primary antibody at a 1:2,000 dilution, overnight at 4° C. For detection HRP conjugated Rb-a-Mouse IgG was used at a 1:20,000 dilution in blocking buffer (p/n MB-070) for 1 h at 4° C followed by visualization using a Biospectrum imaging system (UVP).

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The antibody was serially diluted and tested at a range of concentrations on at least 22 different human formalin-fixed, paraffin archival tissues, and positive and negative tissues were scored and compared to the published literature on the expression and function of the gene. A representative image from positively stained small intestine shows the localization of the anti Pdcd4 antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Image provided courtesy of LifeSpan Biosciences, Seattle, WA