HPA000556

CRIM1 antibody from Atlas Antibodies

S52

Eligible for validation within the Antibodypedia Validation Initiative

Immunohistochemistry

Enhanced validation

Provider product page for HPA000556

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Antibody Data

Product number

HPA000556

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA000556, RRID:AB_611408

Product name

Anti-CRIM1

Provider product page

Antibody type

Polyclonal

Description

Affinity purified using the PrEST antigen as affinity ligand

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

LTEYEAGVCEDENWTDDQLLGFKPCNENLIAGCNI
INGKCECNTIRTCSNPFEFPSQDMCLSALKRIEEE
KPDCSKARCEVQFSPRCPEDSVLIEGYAPPGECCP
LP


Isotype

IgG

Vial size

100 µl

Storage

Store at +4¡C for short term storage. Long time storage is recommended at -20¡C.

References

SOST Inhibits Prostate Cancer Invasion.
Hudson BD, Hum NR, Thomas CB, Kohlgruber A, Sebastian A, Collette NM, Coleman MA, Christiansen BA, Loots GG
PloS one 2015;10(11):e0142058

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Production of a mouse line with a conditional Crim1 mutant allele.
Chiu HS, York JP, Wilkinson L, Zhang P, Little MH, Pennisi DJ
Genesis (New York, N.Y. : 2000) 2012 Sep;50(9):711-6

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CRIM1 is localized to the podocyte filtration slit diaphragm of the adult human kidney.
Nyström J, Hultenby K, Ek S, Sjölund J, Axelson H, Jirström K, Saleem MA, Nilsson K, Johansson ME
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2009 Jul;24(7):2038-44

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From gene expression analysis to tissue microarrays: a rational approach to identify therapeutic and diagnostic targets in lymphoid malignancies.
Ek S, Andréasson U, Hober S, Kampf C, Pontén F, Uhlén M, Merz H, Borrebaeck CA
Molecular & cellular proteomics : MCP 2006 Jun;5(6):1072-81

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human placenta shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows low expression as expected.

Sample type

HUMAN

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human placenta and liver tissues using Anti-CRIM1 antibody. Corresponding CRIM1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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