PA5-34395

antibody from Invitrogen Antibodies

Targeting: TTYH2 C17orf29

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-34395

Provider

Invitrogen Antibodies

Product name

TTYH2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control is human kidney tissue lysate. PA5-34395 can be used with blocking peptide PEP-1437.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

References

Submitted references

SARS-CoV-2 exacerbates proinflammatory responses in myeloid cells through C-type lectin receptors and Tweety family member 2.

Lu Q, Liu J, Zhao S, Gomez Castro MF, Laurent-Rolle M, Dong J, Ran X, Damani-Yokota P, Tang H, Karakousi T, Son J, Kaczmarek ME, Zhang Z, Yeung ST, McCune BT, Chen RE, Tang F, Ren X, Chen X, Hsu JCC, Teplova M, Huang B, Deng H, Long Z, Mudianto T, Jin S, Lin P, Du J, Zang R, Su TT, Herrera A, Zhou M, Yan R, Cui J, Zhu J, Zhou Q, Wang T, Ma J, Koralov SB, Zhang Z, Aifantis I, Segal LN, Diamond MS, Khanna KM, Stapleford KA, Cresswell P, Liu Y, Ding S, Xie Q, Wang J
Immunity 2021 Jun 8;54(6):1304-1319.e9

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TTYH1 and TTYH2 Serve as LRRC8A-Independent Volume-Regulated Anion Channels in Cancer Cells.

Bae Y, Kim A, Cho CH, Kim D, Jung HG, Kim SS, Yoo J, Park JY, Hwang EM
Cells 2019 Jun 9;8(6)

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of TTYH2 in human kidney tissue with TTYH2 Polyclonal Antibody (Product # PA5-34395) at 20 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of TTYH2 in human kidney tissue with TTYH2 Polyclonal Antibody (Product # PA5-34395) at 2.5 µg/mL.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 VRAC activity is completely abolished in dKO cells, and is restored by expression of TTYH1 or TTYH2. ( a ) Western blot data for TTYH1 and LRRC8A (SWELL1) in SNU-601 and TTYH1 and TTYH2 double-knockout (dKO) cells. ( b ) Representative immunocytochemical images of SNU-601 and dKO cells stained with anti-TTYH2 antibody. ( c ) Representative traces of VRAC currents from dKO cells transfected with GFP controls in isotonic and hypotonic solutions. Notably, VRAC currents were not induced by hypotonic stimulation in these cells. ( d ) Representative traces of VRAC currents from dKO cells transfected with TTYH1-GFP in isotonic and hypotonic solutions. ( e ) Representative traces of VRAC currents from dKO cells transfected with TTYH2-GFP in isotonic and hypotonic solutions. ( f ) Representative traces of VRAC currents from dKO cells co-transfected with TTYH1-GFP and TTYH2-GFP in isotonic and hypotonic solutions. ( g ) Summary bar graph showing current densities in isotonic and hypotonic solutions, as in ( c )-( f ), at +100 mV. Data are presented as means +- SEM (** P < 0.01 and *** P < 0.001).