MA5-24851

antibody from Invitrogen Antibodies

Targeting: MMP12 HME

Western blot

Antibody data

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Validation data

Product number: MA5-24851 - Provider product page
Provider: Invitrogen Antibodies
Product name: MMP12 Recombinant Rabbit Monoclonal Antibody (1C11)
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Reactivity: Human, Rat
Host: Rabbit
Isotype: IgG
Antibody clone number: 1C11
Vial size: 100 µL
Concentration: 1.0 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

MMP12 protein structure - MA5-24851 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 2 MMP12 Is Up-regulated in Muscle Tissues and Macrophages of Apc Min/+ Mice. A MMP12 antibody immunostaining in the muscle of healthy individuals. Scale bar: 2 mum. B Immunostaining of MMP12 in muscle tissue of WT mice and Apc Min/+ mice. Scale bar: 5 mum. C Quantification of MM12 expression in gastrocnemius tissue was performed by ImageJ software (40X) (* P < 0.05, data are shown as means +- SD; n = 9 per group). D Representative images of dual immnofluorescent staining of macrophages (F4/80 in green) and MMP12 (in red) in WT mice are shown. The yellow areas in the merged images indicate overlapping localization of the red and green signals, indicated by the white arrows. Scale bars: 20 mum. E Quantification of MMP12 mRNA level in peritoneal macrophages isolated from WT mice and Apc Min/+ mice by qPCR (* P < 0.05; data are shown as the means +- SD; n = 6 per group). F The serum MMP12 levels detected in WT and Apc Min/+ mice at 9, 15, and 24 weeks old by ELISA ( P > 0.05; data are shown as means +- SD; n = 6 per group)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 4 IL-6 Up-regulates MMP12 in Macrophages. A Schematic diagram of tumor cells (MC38/CT26 cell lines) co-cultured with macrophage cells (RAW264.7 cell lines). All quantifications were performed by image J software for gray scale statistics. B , C Representative western blots showing the secreted MMP12 protein levels from RAW264.7 cell lines (1-2 x 10 5 ) cultured alone or co-cultured with MC38 cell lines (control, 1 x 10 4 , 3 x 10 4 , 5 x 10 4 ). beta-Actin as the internal control. D , E Representative western blots showing the secreted MMP12 protein levels from RAW264.7 cell lines (1-2 x 10 5 ) cultured alone or co-cultured with CT26 cell lines (control, 1 x 10 4 , 3 x 10 4 , 5 x 10 4 ). GAPDH as the internal control. F Schematic diagram of IL-6 treated macrophages. RAW264.7 cells incubated with fresh media were served as untreated negative controls. Western blot was used to detect MMP12 in RAW 264.7 cells and GAPDH as the internal control. G , H RAW264.7 cells (1-2 x 10 5 ) were seeded in 6-well plates and treated with increasing doses of IL-6 (0, 2, 5, 10, 30 ng/mL) for 72 h. I Immune Gene data ( https://www.immgen.org/ImmGenApps.html ) suggest that IL-6 receptor expresses on F480+ macrophages. The colored bars refer to the expression level of IL-6 receptors on macrophages. Red represents high expression of IL-6 receptors, and green represents low expression of IL-6 receptors