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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-27254 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MMP12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, H1299, HelaS3. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Agrin-Matrix Metalloproteinase-12 axis confers a mechanically competent microenvironment in skin wound healing.
Chakraborty S, Sampath D, Yu Lin MO, Bilton M, Huang CK, Nai MH, Njah K, Goy PA, Wang CC, Guccione E, Lim CT, Hong W
Nature communications 2021 Nov 3;12(1):6349
Nature communications 2021 Nov 3;12(1):6349
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MMP12 in methanol-fixed H1299 cells using a MMP12 polyclonal antibody (Product # PA5-27254) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using MMP12 (Product # PA5-27254) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of MMP12 was performed in paraffin-embedded human lung adenocarcinoma tissue using MMP12 Polyclonal Antibody (Product # PA5-27254) at a dilution of 1:500.
