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LearnF54484
antibody from NSJ Bioreagents
Targeting: APEX1
APE, APE-1, APEN, APEX, APX, HAP1, REF-1, REF1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- F54484 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- APE1 Antibody / APEX1
- Antibody type
- Monoclonal
- Description
- This highly specific APE1 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence applications with human samples.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 1518CT337.123.86.269.232
- Vial size
- 0.05 ml, 0.2 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the APE1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) A431, 2) HeLa, 3) HepG2, 4) K562 and 5) PC-3 cell lysate with APE1 antibody. Predicted molecular weight ~38 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of fixed and permeabilized human HeLa cells with APE1 antibody (green) and anti-Actin (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human stomach tissue with APE1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human stomach tissue with APE1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
