Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-78150 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RGS7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: U87-MG, SK-N-SH Predicted Reactivity: Mouse (98%), Rat (98%), Chicken (95%), Bovine (98%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RGS7 in whole cell lysate using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with RGS7 polyclonal antibody (Product # PA5-78150) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RGS7 Polyclonal Antibody detects RGS7 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RGS7 Polyclonal Antibody (Product # PA5-78150) diluted by 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RGS7 Polyclonal Antibody detects RGS7 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min. Green: RGS7 protein stained by RGS7 Polyclonal Antibody (Product # PA5-78150) diluted at 1:500. Blue: Hoechst 33342 staining.