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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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- Product number
- AF3135 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal F-Spondin/SPON1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human F-Spondin/SPON1 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references A multiplex immunoassay of serum biomarkers for the detection of uveal melanoma.
Song J, Merbs SL, Sokoll LJ, Chan DW, Zhang Z
Clinical proteomics 2019;16:10
Clinical proteomics 2019;16:10
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human F-Spondin/SPON1 by Western Blot. Western blot shows lysates of OVCAR-3 human ovarian carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human F-Spondin/SPON1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3135) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for F-Spondin/ SPON1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human F-Spondin/SPON1 by Simple WesternTM. Simple Western lane view shows lysates of OVCAR-3 human ovarian carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for F-Spondin/SPON1 at approximately 134 kDa (as indicated) using 10 µg/mL of Goat Anti-Human F-Spondin/SPON1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3135) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
