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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- ELISA [1]
- Immunohistochemistry [3]
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- Product number
- BS-7520R - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SPON1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Antigen: bs-7520P, 2ug/100ul Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1:5000; TMB staining Read the data in Microplate Reader by 450nm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin embedded rat lung labeled with Anti-SPON1/F spondin Polyclonal Antibody, Unconjugated (bs-7520R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SPON1 Polyclonal Antibody, Unconjugated (bs-7520R) at 1:400 overnight at 4°C, DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SPON1 Polyclonal Antibody, Unconjugated (bs-7520R) at 1:400 overnight at 4°C, DAB staining.
