AP6412a

antibody from Abcepta

Targeting: NR4A2 HZF-3, NOT, NURR1, RNR1, TINUR

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP6412a

Provider

Abcepta

Proper citation

Abgent Cat#AP6412a, RRID:AB_2153902

Product name

NURR1 (NR4A2) Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Western blot analysis of anti-NURR1(NR4A2) Pab (Cat. #AP6412a) in mouse brain tissue lysate. NURR1(NR4A2) (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

NR4A2 Antibody (N-term) (Cat. #AP6412a) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NR4A2 antibody detected the NR4A2 protein (arrow).

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of NURR1 (NR4A2) Antibody (N-term) (Cat#AP6412a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Primary Ab dilution

1:10~50

Immunohistochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human brain tissue reacted with NURR1 (NR4A2) antibody (N-term)(Cat#AP6412a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:50~100