PA5-13416

antibody from Invitrogen Antibodies

Targeting: NR4A2 HZF-3, NOT, NURR1, RNR1, TINUR

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-13416

Provider

Invitrogen Antibodies

Product name

Nurr1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine and rat based on sequence homology.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

2.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Characterization and Study of Gene Expression Profiles of Human Periodontal Mesenchymal Stem Cells in Spheroid Cultures by Transcriptome Analysis.

Suga T, Usui M, Onizuka S, Sano K, Sato T, Nakazawa K, Ariyoshi W, Nishihara T, Nakashima K
Stem cells international 2021;2021:5592804

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Age-dependent decrease of Nurr1 protein expression in the gerbil hippocampus.

Ahn JH, Lee JS, Cho JH, Park JH, Lee TK, Song M, Kim H, Kang SH, Won MH, Lee CH
Biomedical reports 2018 Jun;8(6):517-522

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using a NURR1 polyclonal antibody (Product # PA5-13416) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1. Western blot analysis of Nurr1 protein expression in hippocampi derived from young, adult and aged gerbils. The RODs of immunoblot bands are shown as percentage values. Data are presented as the means +- standard error of the mean. *P

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2. Nurr1 immunohistochemistry in hippocampal regions of young, adult and aged gerbils. Nurr1 expression was detected in the (A-C) CA1 and (D-F) CA3 regions and (G-I) DG. In the young group, strong Nurr1 immunoreactivity was detected in pyramidal neurons of the SP in the CA1 and 3 regions (arrows) and in granule cells of the GCL in the DG (arrowheads). Nurr1 immunoreactivity in the SP and GCL gradually decreased in the adult and aged groups. Magnification, x20; scale bar, 100 um. Nurr1, nuclear receptor related-1 protein; DG, dentate gyrus; SO, stratum oriens; SP, stratum pyramidale; SR, stratum radiatum; ML, molecular layer; GCL, granule cell layer; PL, polymorphic layer.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Validation of NR4A2 expression in spheroid cultures of hPDLMSC. (a) Expression of NR4A2 mRNA in monolayer and spheroid-cultured hPDLMSC on day 3 by real-time RT-PCR. * p < 0.05 (compared with monolayer culture). (b) Expression of NR4A2 protein in monolayer and spheroid-cultured hPDLMSC on day 3 by western blotting. (c) NR4A1 and NR4A3 mRNA expression in monolayer and spheroid-cultured hPDLMSC on day 3 by real-time RT-PCR. * p < 0.05 (compared with monolayer culture). All experiments were performed with samples from four donors.