Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
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Validation data
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- Product number
- PA5-22057 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AUTS2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, H1299, HepG2, Raji, NIH-3T3.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AUTS2 using 30 µg of 293T lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an AUTS2 polyclonal antibody (Product # PA5-22057) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of AUTS2 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a AUTS2 Polyclonal Antibody (Product # PA5-22057) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using AUTS2 Polyclonal Antibody (Product # PA5-22057). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3.5% SDS PAGE. AUTS2 Polyclonal Antibody (Product # PA5-22057) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AUTS2 was performed by separating 30 µg of Zebrafish tissue extract by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a AUTS2 Polyclonal Antibody (Product # PA5-22057) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.