- PA5-28686 - Invitrogen Antibodies CSNK2A1 antibody

Submitted references MicroRNA-499 serves as a sensitizer for lung cancer cells to radiotherapy by inhibition of CK2α-mediated phosphorylation of p65.

Ma YS, Shi BW, Lu HM, Xie PF, Xin R, Wu ZJ, Shi Y, Yin YZ, Hou LK, Jia CY, Wu W, Lv ZW, Yu F, Wang GR, Liu JB, Jiang GX, Fu D
Molecular therapy oncolytics 2021 Jun 25;21:171-182

Regulation of the oncogenic phenotype by the nuclear body protein ZC3H8.

Schmidt JA, Danielson KG, Duffner ER, Radecki SG, Walker GT, Shelton A, Wang T, Knepper JE
BMC cancer 2018 Jul 24;18(1):759

BET protein Brd4 activates transcription in neurons and BET inhibitor Jq1 blocks memory in mice.

Korb E, Herre M, Zucker-Scharff I, Darnell RB, Allis CD
Nature neuroscience 2015 Oct;18(10):1464-73

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of CK2 alpha-1 was achieved by transfecting A549 cells with CK2 alpha-1 specific validated siRNAs (Silencer® select Product # s3638). Western blot analysis (Fig. a) was performed using whole cell extracts from the CK2 alpha-1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with CK2 alpha-1 Polyclonal Antibody (Product # PA5-28686, 1:500 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to PA5-28686.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), A-431 (Lane 2), U-87 MG (Lane 3), Jurkat (Lane 4), Hep G2 (Lane 5), HCT 116 (Lane 6), MCF7 (Lane 7) and MKN45 (Lane 8). The blot was probed with Anti-CK2 alpha-1 Polyclonal Antibody (Product # PA5-28686, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 44 kDa band corresponding to CK2 alpha-1 catalytic α-subunits and 26 kDa CK2 alpha-1 regulatory β-subunits was observed across the cell lines tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of CK2-alpha-1 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a CK2 alpha-1 Polyclonal Antibody (Product # PA5-28686) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CK2 alpha-1 was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a CK2 alpha-1 Polyclonal Antibody (Product # PA5-28686) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using CK2 alpha-1 Polyclonal Antibody (Product # PA5-28686). Sample (30 µg whole cell lysate). A: 293T. B: A431. C: H1299. D: HeLa S3. E: HepG2. F: MOLT4. G: Raji. 10% SDS PAGE. CK2 alpha-1 Polyclonal Antibody (Product # PA5-28686) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: CK2 alpha-1 Polyclonal Antibody detects CSNK2A1 protein by western blot analysis. A. 50 µg mouse brain lysate/extract.10% SDS-PAGE. CK2 alpha-1 Polyclonal Antibody (Product # PA5-28686) dilution: 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: CK2 alpha-1 Polyclonal Antibody detects CSNK2A1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CSNK2A1 protein stained by CK2 alpha-1 Polyclonal Antibody (Product # PA5-28686) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Casein Kinase 2 alpha 1 (Product # PA5-28686) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 miR-499 increased the sensitivity of lung cancer A549 cells to IR exposure in vivo (A) Representative pictures of tumors after different treatment. (B) Measurements of tumor volume after overexpression of miR-499 and IR. (C) Measurements of tumor weight after overexpression of miR-499 and IR. (D) H&E staining was conducted to detect the cellular structure changes (x400). (E) Ki67, CK2alpha, and p-p65 expression in tissues detected by IHC. *p < 0.05 versus mice treated with mimic NC; # p < 0.05 versus mice treated with mimic NC + IR, n = 12. The measurement data were expressed as mean +- standard deviation. Data between two groups were compared using independent sample t test. Comparisons among multiple groups were conducted by ANOVA, followed by Bonferroni''s post hoc test.

PA5-28686

Antibody data