A304-959A

antibody from Invitrogen Antibodies

Targeting: RALGAPB DKFZp781M2411, KIAA1219, RalGAPbeta

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

A304-959A

Provider

Invitrogen Antibodies

Product name

RALGAPB Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human RALGAPB by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RALGAPB antibody A304-959A (lot A304-959A-1) used for IP at 6 µg per reaction. RALGAPB was also immunoprecipitated by rabbit anti-RALGAPB antibody BL19420. For blotting immunoprecipitated RALGAPB, A304-959A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse RALGAPB by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RALGAPB antibody A304-959A (lot A304-959A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.