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- Antibody Data
- Antigen structure
- References [30]
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- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 20140-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#20140-1-AP, RRID:AB_10666434
- Product name
- MYH4-Specific antibody
- Antibody type
- Polyclonal
- Description
- MYH4-Specific antibody (Cat. #20140-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, pig and has been validated for the following applications: IHC, WB, ELISA.
- Reactivity
- Human, Mouse, Porcine
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Dietary supplementation with inulin improves burn-induced skeletal muscle atrophy by regulating gut microbiota disorders.
Myogenic exosome miR-140-5p modulates skeletal muscle regeneration and injury repair by regulating muscle satellite cells.
Regulation of myo-miR-24-3p on the Myogenesis and Fiber Type Transformation of Skeletal Muscle.
Bioactive fiber-reinforced hydrogel to tailor cell microenvironment for structural and functional regeneration of myotendinous junction.
Identification of different myofiber types in pigs muscles and construction of regulatory networks.
Pb inhibited C2C12 myoblast differentiation by regulating HDAC2.
"Biqi" Bayberry Extract Promotes Skeletal Muscle Fiber Type Remodeling by Increasing Fast Myofiber Formation via the Akt/FoxO1 Pathway in Mice.
Klotho Null Mutation Involvement in Adenosine A2B Receptor-Related Skeletal Muscle Degeneration.
Endurance Exercise-Induced Fgf21 Promotes Skeletal Muscle Fiber Conversion through TGF-β1 and p38 MAPK Signaling Pathway.
Potential nutritional healthy-aging strategy: enhanced protein metabolism by balancing branched-chain amino acids in a finishing pig model.
Myostain is involved in ginsenoside Rb1-mediated anti-obesity.
Loss of Myostatin Alters Mitochondrial Oxidative Phosphorylation, TCA Cycle Activity, and ATP Production in Skeletal Muscle.
Neuregulin 1 Drives Morphological and Phenotypical Changes in C2C12 Myotubes: Towards De Novo Formation of Intrafusal Fibres In Vitro.
Naringin Promotes Skeletal Muscle Fiber Remodeling by the AdipoR1-APPL1-AMPK Signaling Pathway.
The nuclear envelope protein Net39 is essential for muscle nuclear integrity and chromatin organization.
The long noncoding RNA Meg3 regulates myoblast plasticity and muscle regeneration through epithelial-mesenchymal transition.
MicroRNA-494-3p inhibits formation of fast oxidative muscle fibres by targeting E1A-binding protein p300 in human-induced pluripotent stem cells.
Maternal Methyl-Donor Micronutrient Supplementation During Pregnancy Promotes Skeletal Muscle Differentiation and Maturity in Newborn and Weaning Pigs.
Uncarboxylated osteocalcin decreases insulin-stimulated glucose uptake without affecting insulin signaling and regulators of mitochondrial biogenesis in myotubes.
The phosphatidylcholine transfer protein StarD7 is important for myogenic differentiation in mouse myoblast C2C12 cells and human primary skeletal myoblasts.
Hydrogen sulphide ameliorating skeletal muscle atrophy in db/db mice via Muscle RING finger 1 S-sulfhydration.
Effect of Hypoxia on the Muscle Fiber Switching Signal Pathways CnA/NFATc1 and Myostatin in Mouse Myocytes.
Butyrate promotes slow-twitch myofiber formation and mitochondrial biogenesis in finishing pigs via inducing specific microRNAs and PGC-1α expression1.
Suppression of protein degradation by leucine requires its conversion to β-hydroxy-β-methyl butyrate in C2C12 myotubes.
Antagonism of the thromboxane-prostanoid receptor is cardioprotective against right ventricular pressure overload.
Estrogen/ERR-α signaling axis is associated with fiber-type conversion of upper airway muscles in patients with obstructive sleep apnea hypopnea syndrome.
Accumulation of advanced glycation end-products and activation of the SCAP/SREBP Lipogenetic pathway occur in diet-induced obese mouse skeletal muscle.
Akt1 isoform modulates phenotypic conversion of vascular smooth muscle cells.
Proteomic analysis of skeletal muscle in insulin-resistant mice: response to 6-week aerobic exercise.
MiR-23a inhibits myogenic differentiation through down regulation of fast myosin heavy chain isoforms.
Gao S, Zhao X, Leng Y, Xia Z
Scientific reports 2024 Jan 28;14(1):2328
Scientific reports 2024 Jan 28;14(1):2328
Myogenic exosome miR-140-5p modulates skeletal muscle regeneration and injury repair by regulating muscle satellite cells.
Cao X, Xue L, Yu X, Yan Y, Lu J, Luo X, Wang H, Wang J
Aging 2024 Feb 29;16(5):4609-4630
Aging 2024 Feb 29;16(5):4609-4630
Regulation of myo-miR-24-3p on the Myogenesis and Fiber Type Transformation of Skeletal Muscle.
Fan D, Yao Y, Liu Y, Yan C, Li F, Wang S, Yu M, Xie B, Tang Z
Genes 2024 Feb 21;15(3)
Genes 2024 Feb 21;15(3)
Bioactive fiber-reinforced hydrogel to tailor cell microenvironment for structural and functional regeneration of myotendinous junction.
Sun Y, Sheng R, Cao Z, Liu C, Li J, Zhang P, Du Y, Mo Q, Yao Q, Chen J, Zhang W
Science advances 2024 Apr 26;10(17):eadm7164
Science advances 2024 Apr 26;10(17):eadm7164
Identification of different myofiber types in pigs muscles and construction of regulatory networks.
Li C, Wang Y, Sun X, Yang J, Ren Y, Jia J, Yang G, Liao M, Jin J, Shi X
BMC genomics 2024 Apr 24;25(1):400
BMC genomics 2024 Apr 24;25(1):400
Pb inhibited C2C12 myoblast differentiation by regulating HDAC2.
Gu X, Shen N, Huang C, Wang HL
Toxicology 2023 Nov;499:153639
Toxicology 2023 Nov;499:153639
"Biqi" Bayberry Extract Promotes Skeletal Muscle Fiber Type Remodeling by Increasing Fast Myofiber Formation via the Akt/FoxO1 Pathway in Mice.
Li J, Li Y, Suo X, Li J, Huang D, Kou G
Foods (Basel, Switzerland) 2023 Jun 23;12(13)
Foods (Basel, Switzerland) 2023 Jun 23;12(13)
Klotho Null Mutation Involvement in Adenosine A2B Receptor-Related Skeletal Muscle Degeneration.
Pai HL, Wu PY, Chen DM, Chen ZJ, Yang YS, Chang HH, Lin DP
The American journal of pathology 2023 Jul;193(7):950-959
The American journal of pathology 2023 Jul;193(7):950-959
Endurance Exercise-Induced Fgf21 Promotes Skeletal Muscle Fiber Conversion through TGF-β1 and p38 MAPK Signaling Pathway.
Luo X, Zhang H, Cao X, Yang D, Yan Y, Lu J, Wang X, Wang H
International journal of molecular sciences 2023 Jul 13;24(14)
International journal of molecular sciences 2023 Jul 13;24(14)
Potential nutritional healthy-aging strategy: enhanced protein metabolism by balancing branched-chain amino acids in a finishing pig model.
Zhang L, Guo Q, Duan Y, Wang W, Yang Y, Yin Y, Gong S, Han M, Li F, Yin Y
Food & function 2022 Jun 6;13(11):6217-6232
Food & function 2022 Jun 6;13(11):6217-6232
Myostain is involved in ginsenoside Rb1-mediated anti-obesity.
Li HS, Kuang JY, Liu GJ, Wu WJ, Yin XL, Li HD, Wang L, Qin T, Zhang WC, Sun YY
Pharmaceutical biology 2022 Dec;60(1):1106-1115
Pharmaceutical biology 2022 Dec;60(1):1106-1115
Loss of Myostatin Alters Mitochondrial Oxidative Phosphorylation, TCA Cycle Activity, and ATP Production in Skeletal Muscle.
Wang X, Wei Z, Gu M, Zhu L, Hai C, Di A, Wu D, Bai C, Su G, Liu X, Yang L, Li G
International journal of molecular sciences 2022 Dec 11;23(24)
International journal of molecular sciences 2022 Dec 11;23(24)
Neuregulin 1 Drives Morphological and Phenotypical Changes in C2C12 Myotubes: Towards De Novo Formation of Intrafusal Fibres In Vitro.
Barrett P, Quick TJ, Mudera V, Player DJ
Frontiers in cell and developmental biology 2021;9:760260
Frontiers in cell and developmental biology 2021;9:760260
Naringin Promotes Skeletal Muscle Fiber Remodeling by the AdipoR1-APPL1-AMPK Signaling Pathway.
Li P, Zhang S, Song H, Traore SS, Li J, Raubenheimer D, Cui Z, Kou G
Journal of agricultural and food chemistry 2021 Oct 13;69(40):11890-11899
Journal of agricultural and food chemistry 2021 Oct 13;69(40):11890-11899
The nuclear envelope protein Net39 is essential for muscle nuclear integrity and chromatin organization.
Ramirez-Martinez A, Zhang Y, Chen K, Kim J, Cenik BK, McAnally JR, Cai C, Shelton JM, Huang J, Brennan A, Evers BM, Mammen PPA, Xu L, Bassel-Duby R, Liu N, Olson EN
Nature communications 2021 Jan 29;12(1):690
Nature communications 2021 Jan 29;12(1):690
The long noncoding RNA Meg3 regulates myoblast plasticity and muscle regeneration through epithelial-mesenchymal transition.
Dill TL, Carroll A, Pinheiro A, Gao J, Naya FJ
Development (Cambridge, England) 2021 Jan 15;148(2)
Development (Cambridge, England) 2021 Jan 15;148(2)
MicroRNA-494-3p inhibits formation of fast oxidative muscle fibres by targeting E1A-binding protein p300 in human-induced pluripotent stem cells.
Iwasaki H, Ichihara Y, Morino K, Lemecha M, Sugawara L, Sawano T, Miake J, Sakurai H, Nishi E, Maegawa H, Imamura T
Scientific reports 2021 Jan 13;11(1):1161
Scientific reports 2021 Jan 13;11(1):1161
Maternal Methyl-Donor Micronutrient Supplementation During Pregnancy Promotes Skeletal Muscle Differentiation and Maturity in Newborn and Weaning Pigs.
He Q, Zou T, Chen J, Jian L, He J, Xia Y, Xie F, Wang Z, You J
Frontiers in nutrition 2020;7:609022
Frontiers in nutrition 2020;7:609022
Uncarboxylated osteocalcin decreases insulin-stimulated glucose uptake without affecting insulin signaling and regulators of mitochondrial biogenesis in myotubes.
Parry HA, Rivera ME, Vaughan RA, Sunderland KL
Journal of physiology and biochemistry 2020 Feb;76(1):169-178
Journal of physiology and biochemistry 2020 Feb;76(1):169-178
The phosphatidylcholine transfer protein StarD7 is important for myogenic differentiation in mouse myoblast C2C12 cells and human primary skeletal myoblasts.
Horibata Y, Mitsuhashi S, Shimizu H, Maejima S, Sakamoto H, Aoyama C, Ando H, Sugimoto H
Scientific reports 2020 Feb 18;10(1):2845
Scientific reports 2020 Feb 18;10(1):2845
Hydrogen sulphide ameliorating skeletal muscle atrophy in db/db mice via Muscle RING finger 1 S-sulfhydration.
Lu F, Lu B, Zhang L, Wen J, Wang M, Zhang S, Li Q, Shu F, Sun Y, Liu N, Peng S, Zhao Y, Dong S, Zhao D, Lu F, Zhang W
Journal of cellular and molecular medicine 2020 Aug;24(16):9362-9377
Journal of cellular and molecular medicine 2020 Aug;24(16):9362-9377
Effect of Hypoxia on the Muscle Fiber Switching Signal Pathways CnA/NFATc1 and Myostatin in Mouse Myocytes.
Lixin L, Juan W, Yun B, Jingwei L, Xiuju Y, Xiaomao L, Zhiwei Z, Xiaoyan H, Yanjun D, Hongquan L, Haidong W
Acta histochemica 2019 Jul;121(5):539-545
Acta histochemica 2019 Jul;121(5):539-545
Butyrate promotes slow-twitch myofiber formation and mitochondrial biogenesis in finishing pigs via inducing specific microRNAs and PGC-1α expression1.
Zhang Y, Yu B, Yu J, Zheng P, Huang Z, Luo Y, Luo J, Mao X, Yan H, He J, Chen D
Journal of animal science 2019 Jul 30;97(8):3180-3192
Journal of animal science 2019 Jul 30;97(8):3180-3192
Suppression of protein degradation by leucine requires its conversion to β-hydroxy-β-methyl butyrate in C2C12 myotubes.
Duan Y, Zhong Y, Song B, Zheng C, Xu K, Kong X, Li F
Aging 2019 Dec 24;11(24):11922-11936
Aging 2019 Dec 24;11(24):11922-11936
Antagonism of the thromboxane-prostanoid receptor is cardioprotective against right ventricular pressure overload.
West JD, Voss BM, Pavliv L, de Caestecker M, Hemnes AR, Carrier EJ
Pulmonary circulation 2016 Jun;6(2):211-23
Pulmonary circulation 2016 Jun;6(2):211-23
Estrogen/ERR-α signaling axis is associated with fiber-type conversion of upper airway muscles in patients with obstructive sleep apnea hypopnea syndrome.
Chen HH, Lu J, Guan YF, Li SJ, Hu TT, Xie ZS, Wang F, Peng XH, Liu X, Xu X, Zhao FP, Yu BL, Li XP
Scientific reports 2016 Jun 2;6:27088
Scientific reports 2016 Jun 2;6:27088
Accumulation of advanced glycation end-products and activation of the SCAP/SREBP Lipogenetic pathway occur in diet-induced obese mouse skeletal muscle.
Mastrocola R, Collino M, Nigro D, Chiazza F, D'Antona G, Aragno M, Minetto MA
PloS one 2015;10(3):e0119587
PloS one 2015;10(3):e0119587
Akt1 isoform modulates phenotypic conversion of vascular smooth muscle cells.
Yun SJ, Ha JM, Kim EK, Kim YW, Jin SY, Lee DH, Song SH, Kim CD, Shin HK, Bae SS
Biochimica et biophysica acta 2014 Nov;1842(11):2184-92
Biochimica et biophysica acta 2014 Nov;1842(11):2184-92
Proteomic analysis of skeletal muscle in insulin-resistant mice: response to 6-week aerobic exercise.
Yuan H, Niu Y, Liu X, Yang F, Niu W, Fu L
PloS one 2013;8(1):e53887
PloS one 2013;8(1):e53887
MiR-23a inhibits myogenic differentiation through down regulation of fast myosin heavy chain isoforms.
Wang L, Chen X, Zheng Y, Li F, Lu Z, Chen C, Liu J, Wang Y, Peng Y, Shen Z, Gao J, Zhu M, Chen H
Experimental cell research 2012 Nov 1;318(18):2324-34
Experimental cell research 2012 Nov 1;318(18):2324-34
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20140-1-AP(MYH4-Specific antibody) at dilution of 1:300
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using MYH4 antibody 20140-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human kidney using 20140-1-AP(MYH4-Specific antibody) at dilution of 1:200 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human kidney using 20140-1-AP(MYH4-Specific antibody) at dilution of 1:200 (under 40x lens)
- Sample type
- tissue
