20140-1-AP

antibody from Proteintech Group

Targeting: MYH4 MYH2B, MyHC-2B, MyHC-IIb

Provider product page for 20140-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [48]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 20140-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#20140-1-AP, RRID:AB_10666434
Product name: MYH4-Specific antibody
Antibody type: Polyclonal
Description: MYH4-Specific antibody (Cat. #20140-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, pig and has been validated for the following applications: IHC, WB, ELISA.
Reactivity: Human, Mouse, Porcine
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

MYH4 protein structure - 20140-1-AP shown in red.

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Loss of STARD7 Impairs Mitochondrial Phospholipid Homeostasis and Contributes to Mitochondrial Myopathy.

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FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2026 Apr 30;40(8):e71777

Areca nut extract exposure disrupts myogenesis and metabolism in C2C12 cells.

Chen CW, Huang TT, Chen YR, Liu CH, Hsieh CH, Chen TL, Lai KC
Current research in toxicology 2025;9:100264

WNT5B regulates myogenesis and fiber type conversion by affecting mRNA stability.

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Mechanisms of skeletal muscle atrophy in type 2 diabetes mellitus.

Yang J, Wang Y, Xu Y, Jia X, Lu F
Frontiers in physiology 2025;16:1607873

Indole-3-propionic acid enhances glycolytic myofiber formation in piglets through PI3K-mTOR activation and gut microbiota-driven tryptophan metabolic alteration.

Kong Y, Wang Q, Wang J, Qiu X, Yang Y, Liu J, Yang F, Qi R
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Melatonin Activates AMPK Pathway to Regulate the Regeneration of Slow Muscle Fibers in Skeletal Muscle Injury Repair.

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Cold Stress Regulates Muscle Development and Promotes Muscle Fiber Transformation by Regulating Mib1/Notch Pathway.

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GPCPD1 is regulated by METTL3/IGF2BP3 dependent m(6)A modification to affect pig muscle fiber type conversion.

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O-GlcNAcylation modification of MyoD regulates skeletal muscle fiber differentiation by antagonizing the UPF1 pathway.

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The Journal of biological chemistry 2025 Apr;301(4):108364

Light-phase time-restricted feeding disrupts the muscle clock and insulin sensitivity yet potentially induces muscle fiber remodeling in mice.

Ye Z, Huang K, Dai X, Gao D, Gu Y, Qian J, Zhang F, Zhai Q
Heliyon 2024 Sep 30;10(18):e37475

Endurance exercise-induced histone methylation modification involved in skeletal muscle fiber type transition and mitochondrial biogenesis.

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Tangeretin Enhances Muscle Endurance and Aerobic Metabolism in Mice via Targeting AdipoR1 to Increase Oxidative Myofibers.

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Dietary supplementation with inulin improves burn-induced skeletal muscle atrophy by regulating gut microbiota disorders.

Gao S, Zhao X, Leng Y, Xia Z
Scientific reports 2024 Jan 28;14(1):2328

Myogenic exosome miR-140-5p modulates skeletal muscle regeneration and injury repair by regulating muscle satellite cells.

Cao X, Xue L, Yu X, Yan Y, Lu J, Luo X, Wang H, Wang J
Aging 2024 Feb 29;16(5):4609-4630

Regulation of myo-miR-24-3p on the Myogenesis and Fiber Type Transformation of Skeletal Muscle.

Fan D, Yao Y, Liu Y, Yan C, Li F, Wang S, Yu M, Xie B, Tang Z
Genes 2024 Feb 21;15(3)

Comparative Analysis of Muscle Fibers in Selected Muscles of Working and Companion Dog Breeds.

Osiak-Wicha C, Kras K, Arciszewski MB
Animals : an open access journal from MDPI 2024 Dec 11;14(24)

Inhibition of Sesn2 has negative regulatory effects on the myogenic differentiation of C2C12 myoblasts.

Song Z, Lin Q, Liang J, Zhang W
Molecular biomedicine 2024 Aug 9;5(1):31

Bioactive fiber-reinforced hydrogel to tailor cell microenvironment for structural and functional regeneration of myotendinous junction.

Sun Y, Sheng R, Cao Z, Liu C, Li J, Zhang P, Du Y, Mo Q, Yao Q, Chen J, Zhang W
Science advances 2024 Apr 26;10(17):eadm7164

Identification of different myofiber types in pigs muscles and construction of regulatory networks.

Li C, Wang Y, Sun X, Yang J, Ren Y, Jia J, Yang G, Liao M, Jin J, Shi X
BMC genomics 2024 Apr 24;25(1):400

Pb inhibited C2C12 myoblast differentiation by regulating HDAC2.

Gu X, Shen N, Huang C, Wang HL
Toxicology 2023 Nov;499:153639

"Biqi" Bayberry Extract Promotes Skeletal Muscle Fiber Type Remodeling by Increasing Fast Myofiber Formation via the Akt/FoxO1 Pathway in Mice.

Li J, Li Y, Suo X, Li J, Huang D, Kou G
Foods (Basel, Switzerland) 2023 Jun 23;12(13)

Klotho Null Mutation Involvement in Adenosine A2B Receptor-Related Skeletal Muscle Degeneration.

Pai HL, Wu PY, Chen DM, Chen ZJ, Yang YS, Chang HH, Lin DP
The American journal of pathology 2023 Jul;193(7):950-959

Endurance Exercise-Induced Fgf21 Promotes Skeletal Muscle Fiber Conversion through TGF-β1 and p38 MAPK Signaling Pathway.

Luo X, Zhang H, Cao X, Yang D, Yan Y, Lu J, Wang X, Wang H
International journal of molecular sciences 2023 Jul 13;24(14)

Potential nutritional healthy-aging strategy: enhanced protein metabolism by balancing branched-chain amino acids in a finishing pig model.

Zhang L, Guo Q, Duan Y, Wang W, Yang Y, Yin Y, Gong S, Han M, Li F, Yin Y
Food & function 2022 Jun 6;13(11):6217-6232

Myostain is involved in ginsenoside Rb1-mediated anti-obesity.

Li HS, Kuang JY, Liu GJ, Wu WJ, Yin XL, Li HD, Wang L, Qin T, Zhang WC, Sun YY
Pharmaceutical biology 2022 Dec;60(1):1106-1115

Loss of Myostatin Alters Mitochondrial Oxidative Phosphorylation, TCA Cycle Activity, and ATP Production in Skeletal Muscle.

Wang X, Wei Z, Gu M, Zhu L, Hai C, Di A, Wu D, Bai C, Su G, Liu X, Yang L, Li G
International journal of molecular sciences 2022 Dec 11;23(24)

Neuregulin 1 Drives Morphological and Phenotypical Changes in C2C12 Myotubes: Towards De Novo Formation of Intrafusal Fibres In Vitro.

Barrett P, Quick TJ, Mudera V, Player DJ
Frontiers in cell and developmental biology 2021;9:760260

Naringin Promotes Skeletal Muscle Fiber Remodeling by the AdipoR1-APPL1-AMPK Signaling Pathway.

Li P, Zhang S, Song H, Traore SS, Li J, Raubenheimer D, Cui Z, Kou G
Journal of agricultural and food chemistry 2021 Oct 13;69(40):11890-11899

The nuclear envelope protein Net39 is essential for muscle nuclear integrity and chromatin organization.

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The long noncoding RNA Meg3 regulates myoblast plasticity and muscle regeneration through epithelial-mesenchymal transition.

Dill TL, Carroll A, Pinheiro A, Gao J, Naya FJ
Development (Cambridge, England) 2021 Jan 15;148(2)

MicroRNA-494-3p inhibits formation of fast oxidative muscle fibres by targeting E1A-binding protein p300 in human-induced pluripotent stem cells.

Iwasaki H, Ichihara Y, Morino K, Lemecha M, Sugawara L, Sawano T, Miake J, Sakurai H, Nishi E, Maegawa H, Imamura T
Scientific reports 2021 Jan 13;11(1):1161

Maternal Methyl-Donor Micronutrient Supplementation During Pregnancy Promotes Skeletal Muscle Differentiation and Maturity in Newborn and Weaning Pigs.

He Q, Zou T, Chen J, Jian L, He J, Xia Y, Xie F, Wang Z, You J
Frontiers in nutrition 2020;7:609022

Uncarboxylated osteocalcin decreases insulin-stimulated glucose uptake without affecting insulin signaling and regulators of mitochondrial biogenesis in myotubes.

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Journal of physiology and biochemistry 2020 Feb;76(1):169-178

The phosphatidylcholine transfer protein StarD7 is important for myogenic differentiation in mouse myoblast C2C12 cells and human primary skeletal myoblasts.

Horibata Y, Mitsuhashi S, Shimizu H, Maejima S, Sakamoto H, Aoyama C, Ando H, Sugimoto H
Scientific reports 2020 Feb 18;10(1):2845

Hydrogen sulphide ameliorating skeletal muscle atrophy in db/db mice via Muscle RING finger 1 S-sulfhydration.

Lu F, Lu B, Zhang L, Wen J, Wang M, Zhang S, Li Q, Shu F, Sun Y, Liu N, Peng S, Zhao Y, Dong S, Zhao D, Lu F, Zhang W
Journal of cellular and molecular medicine 2020 Aug;24(16):9362-9377

Effect of Hypoxia on the Muscle Fiber Switching Signal Pathways CnA/NFATc1 and Myostatin in Mouse Myocytes.

Lixin L, Juan W, Yun B, Jingwei L, Xiuju Y, Xiaomao L, Zhiwei Z, Xiaoyan H, Yanjun D, Hongquan L, Haidong W
Acta histochemica 2019 Jul;121(5):539-545

Butyrate promotes slow-twitch myofiber formation and mitochondrial biogenesis in finishing pigs via inducing specific microRNAs and PGC-1α expression1.

Zhang Y, Yu B, Yu J, Zheng P, Huang Z, Luo Y, Luo J, Mao X, Yan H, He J, Chen D
Journal of animal science 2019 Jul 30;97(8):3180-3192

Suppression of protein degradation by leucine requires its conversion to β-hydroxy-β-methyl butyrate in C2C12 myotubes.

Duan Y, Zhong Y, Song B, Zheng C, Xu K, Kong X, Li F
Aging 2019 Dec 24;11(24):11922-11936

Dietary leucine supplementation alters energy metabolism and induces slow-to-fast transitions in longissimus dorsi muscle of weanling piglets.

Fan Q, Long B, Yan G, Wang Z, Shi M, Bao X, Hu J, Li X, Chen C, Zheng Z, Yan X
The British journal of nutrition 2017 May;117(9):1222-1234

Antagonism of the thromboxane-prostanoid receptor is cardioprotective against right ventricular pressure overload.

West JD, Voss BM, Pavliv L, de Caestecker M, Hemnes AR, Carrier EJ
Pulmonary circulation 2016 Jun;6(2):211-23

Estrogen/ERR-α signaling axis is associated with fiber-type conversion of upper airway muscles in patients with obstructive sleep apnea hypopnea syndrome.

Chen HH, Lu J, Guan YF, Li SJ, Hu TT, Xie ZS, Wang F, Peng XH, Liu X, Xu X, Zhao FP, Yu BL, Li XP
Scientific reports 2016 Jun 2;6:27088

Accumulation of advanced glycation end-products and activation of the SCAP/SREBP Lipogenetic pathway occur in diet-induced obese mouse skeletal muscle.

Mastrocola R, Collino M, Nigro D, Chiazza F, D'Antona G, Aragno M, Minetto MA
PloS one 2015;10(3):e0119587

Akt1 isoform modulates phenotypic conversion of vascular smooth muscle cells.

Yun SJ, Ha JM, Kim EK, Kim YW, Jin SY, Lee DH, Song SH, Kim CD, Shin HK, Bae SS
Biochimica et biophysica acta 2014 Nov;1842(11):2184-92

Proteomic analysis of skeletal muscle in insulin-resistant mice: response to 6-week aerobic exercise.

Yuan H, Niu Y, Liu X, Yang F, Niu W, Fu L
PloS one 2013;8(1):e53887

MiR-23a inhibits myogenic differentiation through down regulation of fast myosin heavy chain isoforms.

Wang L, Chen X, Zheng Y, Li F, Lu Z, Chen C, Liu J, Wang Y, Peng Y, Shen Z, Gao J, Zhu M, Chen H
Experimental cell research 2012 Nov 1;318(18):2324-34

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20140-1-AP(MYH4-Specific antibody) at dilution of 1:300
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using MYH4 antibody 20140-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human kidney using 20140-1-AP(MYH4-Specific antibody) at dilution of 1:200 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human kidney using 20140-1-AP(MYH4-Specific antibody) at dilution of 1:200 (under 40x lens)
Sample type: tissue