14-9773-80

antibody from Invitrogen Antibodies

Targeting: SOX11

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14-9773-80

Provider

Invitrogen Antibodies

Product name

Anti-SOX11 Monoclonal Antibody (SOX11-C1), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The monoclonal antibody SOX11-C1 (C1) recognizes human Sox11. The transcription factor Sox11 is a member of the SOX (sex determining region Y-related HMG (High Mobility Group) Box) family of proteins. Under normal conditions Sox11 is expressed in the developing central nervous system during embryogenesis and has sequence homology to Sox4. Sox11 plays a role in neuronal maturation and epithelial-mesenchymal interactions and is required for the survival of neuronal and mesenchymal progenitor cells. Sox11 is expressed in mantle cell lymphoma (MCL) and in subsets of hairy cell leukemias, Burkitt lymphomas, and B cell lymphoblastic leukemias, but is not expressed in other B cell lymphomas or in normal B lymphocytes. Sox11 is also expressed in epithelial ovarian cancer and gliomas. Applications Reported: This SOX11-C1 antibody has been reported for use in flow cytometric analysis, western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry. Applications Tested: This SOX11-C1 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low or high pH antigen retrieval. The antibody can be used at less than or equal to 5 µg/mL. This SOX11-C1 antibody has been tested by immunocytochemistry of fixed and permeabilized cells and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

SOX11-C1

Vial size

25 µg

Concentration

0.5 mg/mL

Storage

4° C

References

Submitted references

The role of SOX11 in mantle cell lymphoma.

Lu TX, Li JY, Xu W
Leukemia research 2013 Nov;37(11):1412-9

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Expanded clinical and experimental use of SOX11 - using a monoclonal antibody.

Nordström L, Andréasson U, Jerkeman M, Dictor M, Borrebaeck C, Ek S
BMC cancer 2012 Jun 27;12:269

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Nuclear expression of the non B-cell lineage Sox11 transcription factor identifies mantle cell lymphoma.

Ek S, Dictor M, Jerkeman M, Jirström K, Borrebaeck CA
Blood 2008 Jan 15;111(2):800-5

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Expression of the Sox11 gene in mouse embryos suggests roles in neuronal maturation and epithelio-mesenchymal induction.

Hargrave M, Wright E, Kun J, Emery J, Cooper L, Koopman P
Developmental dynamics : an official publication of the American Association of Anatomists 1997 Oct;210(2):79-86

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of SOX11 was achieved by transfecting SH-SY5Y with SOX11 specific siRNAs (Silencer® select Product # s224668, s194808) . Western blot analysis (Fig. a) was performed using modified whole cell extracts (1% SDS) from the SOX11 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with SOX11 Monoclonal Antibody (SOX11-C1), eBioscience™ (Product # 14-9773-82, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown i histogram (Fig. b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to SOX11.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using SOX11 Monoclonal Antibody (SOX11-C1), eBioscience™ (Product # 14-9773-82) and a 55 kDa band corresponding to SOX11 was observed across cell lines tested except BeWo and HL-60 which are reported to be negative. Whole cell extracts (30 µg lysate) of SHSY-5Y (Lane 1), IMR-32 (Lane 2), BeWo (Lane 3) HL-60 (Lane 4) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry of fixed and permeabilized HepG2 cells using 10 µg/mL of Anti-Human Sox11 Purified followed by F (ab')2 Anti-Mouse IgG eFluor® 570.Nuclei are stained with DAPI, colocalization appears pink.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of formalin-fixed paraffin embedded human Mantle Cell lymphoma using 10 µg/mL of Anti-Human Sox11 Purified followed by Anti-Mouse IgG Biotin, Streptavidin HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of SOX11 was performed using 70% confluent log phase SHSY-5Y and BeWo cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SOX11 Monoclonal Antibody (SOX11-C1), eBioscience™ (Product # 14-9773-82) at 5µg/ml concentration in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization to nucleus. Panel e shows BeWo cells with no expression of SOX11. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.