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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Immunocytochemistry [3]
- Other assay [4]
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- Product number
- PA1-846 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SRC2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-846 detects glucocorticoid receptor interacting protein-1 (GRIP1) from hamster and human cells. PA1-846 has been successfully used in immunocytochemistry, and immunofluorescence procedures. Immunocytochemical staining of GRIP1 in CHO cells with PA1-846 results in cytoplasmic and nuclear staining. This antibody has not been used successfully in Western blot procedures. PA1-846 immunogen is a synthetic peptide corresponding to residues P(206) L P D S E E E G H D S(217) C of human GRIP1. The PA1-846 immunizing peptide (Cat. # PEP-050) is available for use in neutralization and control experiments.
- Reactivity
- Human, Hamster
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Regulation of GRIP1 and CBP Coactivator activity by Rho GDI modulates estrogen receptor transcriptional enhancement.
Factor recruitment and TIF2/GRIP1 corepressor activity at a collagenase-3 response element that mediates regulation by phorbol esters and hormones.
Multiple receptor interaction domains of GRIP1 function in synergy.
Multiple receptor interaction domains of GRIP1 function in synergy.
GRIP1, a transcriptional coactivator for the AF-2 transactivation domain of steroid, thyroid, retinoid, and vitamin D receptors.
GRIP1, a transcriptional coactivator for the AF-2 transactivation domain of steroid, thyroid, retinoid, and vitamin D receptors.
Su LF, Wang Z, Garabedian MJ
The Journal of biological chemistry 2002 Oct 4;277(40):37037-44
The Journal of biological chemistry 2002 Oct 4;277(40):37037-44
Factor recruitment and TIF2/GRIP1 corepressor activity at a collagenase-3 response element that mediates regulation by phorbol esters and hormones.
Rogatsky I, Zarember KA, Yamamoto KR
The EMBO journal 2001 Nov 1;20(21):6071-83
The EMBO journal 2001 Nov 1;20(21):6071-83
Multiple receptor interaction domains of GRIP1 function in synergy.
Schmidt S, Baniahmad A, Eggert M, Schneider S, Renkawitz R
Nucleic acids research 1998 Mar 1;26(5):1191-7
Nucleic acids research 1998 Mar 1;26(5):1191-7
Multiple receptor interaction domains of GRIP1 function in synergy.
Schmidt S, Baniahmad A, Eggert M, Schneider S, Renkawitz R
Nucleic acids research 1998 Mar 1;26(5):1191-7
Nucleic acids research 1998 Mar 1;26(5):1191-7
GRIP1, a transcriptional coactivator for the AF-2 transactivation domain of steroid, thyroid, retinoid, and vitamin D receptors.
Hong H, Kohli K, Garabedian MJ, Stallcup MR
Molecular and cellular biology 1997 May;17(5):2735-44
Molecular and cellular biology 1997 May;17(5):2735-44
GRIP1, a transcriptional coactivator for the AF-2 transactivation domain of steroid, thyroid, retinoid, and vitamin D receptors.
Hong H, Kohli K, Garabedian MJ, Stallcup MR
Molecular and cellular biology 1997 May;17(5):2735-44
Molecular and cellular biology 1997 May;17(5):2735-44
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunolocalization of GRIP1 in CHO cells using Product # PA1-846.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of GRIP1 was done on 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GRIP1 Rabbit Polyclonal Antibody (Product # PA1-846) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunolocalization of GRIP1 in CHO cells using Product # PA1-846.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
