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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NB100-388 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-388, RRID:AB_10077919
- Product name
- Rabbit Polyclonal NCOA2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Antibody was affinity purified using an epitope specific to SRC2 immobilized on solid support.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Antigen sequence
The epitope recognized by NB 100-38
8 maps to a region between residues
700 and 750 of human Nuclear Recep
tor Coactivator 2 using the numberi
ng given in entry NP_006531.1 (Gene
ID 10499).- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Hec1 Tail Phosphorylation Differentially Regulates Mammalian Kinetochore Coupling to Polymerizing and Depolymerizing Microtubules.
Long AF, Udy DB, Dumont S
Current biology : CB 2017 Jun 5;27(11):1692-1699.e3
Current biology : CB 2017 Jun 5;27(11):1692-1699.e3
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NCOA2 Antibody [NB100-388] - Detection of Human SRC2 on HeLa whole cell lysate using NB100-388.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NCOA2 Antibody [NB100-388] - Detection of Mouse NCOA2/ SRC2 by Western Blot. Samples: Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-NCOA2/SRC2 antibody NB100-388 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: NCOA2 Antibody [NB100-388] - Detection of human NCOA2/ SRC2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa(15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NCOA2/SRC2 antibody NB100-388 (lot 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. SRC2 was also immunoprecipitated by a previous lot (lot 1) and by rabbit anti-NCOA1/SRC2 antibody NBP2-22162, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
