PA5-35145

antibody from Invitrogen Antibodies

Targeting: FOXD1 FKHL8, FREAC4

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-35145

Provider

Invitrogen Antibodies

Product name

FOXD1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with mouse based on sequence homology.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

1.7 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

FOXD1 facilitates pancreatic cancer cell proliferation, invasion, and metastasis by regulating GLUT1-mediated aerobic glycolysis.

Cai K, Chen S, Zhu C, Li L, Yu C, He Z, Sun C
Cell death & disease 2022 Sep 3;13(9):765

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of FOXD1 showing staining in the nucleus of B-3 cells using a FOXD1 polyclonal antibody (Product # PA5-35145) followed by detection using a fluorescent conjugated secondary antibody (green). Cytoplasmic actin was stained with a fluorescent red phalloidin (7units/mL, 1 h at 37ºC).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 1 FOXD1 is overexpressed in PC tissues and cells. A The intersection between differentially expressed genes (DEGs) in GSE16515 and FOX family members, represented as a Venn diagram. B Volcano map of the DEGs in GSE16515. C Overall survival curve and disease-free survival curve of FOXD1 differentially expressed PC patients based on TCGA database. D Relative expression of FOXD1 in 179 PC(T) tissues and 171 non-tumor (N) tissues based on TCGA and GTEx database. E GSEA analysis of FOXD1 expression-related genes. F Relative FOXD1 expression in 40 pairs of PC (T) tissues and non-tumor (N) tissues detected by RT-qPCR. G Relative FOXD1 expression in 90 pairs PC (T) tissues and non-tumor (N) tissues detected by immunohistochemistry (IHC). H Overall survival curve of 90 PC patients classified by IHC score. I Relative FOXD1 expression in HPNE cells and PC cells detect by RT-qPCR. J Relative FOXD1 expression in HPNE cells and PC cells detect by western blotting analysis. * p < 0.05; ** p < 0.01.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 3 FOXD1 promotes PC cell proliferation and metastasis in vivo. A Excised subcutaneous tumors 30 days after inoculation with the indicated PANC-1 cells. B Growth curves of subcutaneous tumor volume in each group. C Weight of subcutaneous tumors in each group. D Representative images of FOXD1, KI67, PCNA and GLUT1 expression in subcutaneous tumors detected through IHC. E Representative images of pulmonary metastases in each group. * p < 0.05; ** p < 0.01.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 7 FOXD1 expression correlates with GLUT1 in PC. A Representative image of FOXD1, HOXA11-AS, miR-148b-3p, and GLUT1 expression in serial sections of pancreatic cancer specimens detected through IHC and ISH. Pearson's chi-squared test of the expression of 40 specimens. B GLUT1 expression of MIA PaCa-2 and PANC-1 cells with FOXD1 overexpression and knock-down were detected using western blotting. C Illustration of how FOXD1 facilitates pancreatic cancer cell proliferation, invasion, and metastasis by regulating GLUT1-mediated aerobic glycolysis, created by Figdraw ( http://www.figdraw.com ). * p < 0.05; ** p < 0.01.