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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-72970 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FAT4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The TCM Prescription Yi-Fei-Jie-Du-Tang Inhibit Invasive Migration and EMT of Lung Cancer Cells by Activating Autophagy.
Tumor suppressive role of mitochondrial sirtuin 4 in induction of G2/M cell cycle arrest and apoptosis in hepatitis B virus-related hepatocellular carcinoma.
Wang S, Wang Z, Wu Y, Hou C, Dai X, Wang Q, Wu Y, Qian C, Zhang X
Evidence-based complementary and alternative medicine : eCAM 2022;2022:9160616
Evidence-based complementary and alternative medicine : eCAM 2022;2022:9160616
Tumor suppressive role of mitochondrial sirtuin 4 in induction of G2/M cell cycle arrest and apoptosis in hepatitis B virus-related hepatocellular carcinoma.
Huang FY, Wong DK, Seto WK, Mak LY, Cheung TT, Yuen MF
Cell death discovery 2021 Apr 30;7(1):88
Cell death discovery 2021 Apr 30;7(1):88
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of FAT4 in mouse prostate cancer. Samples were incubated with FAT4 polyclonal antibody (Product # PA5-72970) followed by DAB with hematoxylin counterstain.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 7 SIRT4 overexpression has anti-proliferative effects on liver cancer cells. A and B Flow cytometry analysis of HepG2-HBx and HepG2.2.15 cells after transfection with SIRT4 plasmid for 24, 48, and 72 h, followed by staining with annexin V-FITC and PI. The percentage of apoptotic cells was increased with time. C and D Flow cytometry analysis of cell cycle distribution indicating SIRT4 overexpression induces cell-cycle arrest at G2/M. E Western blot-analysis of cell-cycle-related proteins expression at 48 h after SIRT4 transfection. F Representative fluorescence images showing the retention of Cyclin B1 in the cytoplasm after SIRT4 overexpression. The quantitative data shown are mean +- SEM of triplicate samples. * p < 0.01 and *** p < 0.0001.
