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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00023049-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00023049-M02, RRID:AB_922476
- Product name
- Mouse Monoclonal SMG1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. SMG1 (1A8)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references The nonsense-mediated mRNA decay SMG-1 kinase is regulated by large-scale conformational changes controlled by SMG-8.
Processing bodies are not required for mammalian nonsense-mediated mRNA decay.
Arias-Palomo E, Yamashita A, Fernández IS, Núñez-Ramírez R, Bamba Y, Izumi N, Ohno S, Llorca O
Genes & development 2011 Jan 15;25(2):153-64
Genes & development 2011 Jan 15;25(2):153-64
Processing bodies are not required for mammalian nonsense-mediated mRNA decay.
Stalder L, Mühlemann O
RNA (New York, N.Y.) 2009 Jul;15(7):1265-73
RNA (New York, N.Y.) 2009 Jul;15(7):1265-73
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMG1 Antibody (1A8) [H00023049-M02] - SMG1 monoclonal antibody (M02), clone 1A8 Analysis of SMG1 expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: SMG1 Antibody (1A8) [H00023049-M02] - Detection limit for recombinant GST tagged SMG1 is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SMG1 Antibody (1A8) [H00023049-M02] - Analysis of monoclonal antibody to SMG1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.
