Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-2321 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2321, RRID:AB_2192940
- Product name
- Rabbit Polyclonal SMG1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMG1 Antibody [NB100-2321] - Detection of Human SMG1 on HeLa whole cell lysate using NB100-2321. IP of SMG1 was also successful when using another affinity purified rabbit anti-SMG1 antibody but not when using NB100-2320.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMG1 Antibody [NB100-2321] - Detection of human SMG1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMG1 antibody NB100-2321 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SMG1 Antibody [NB100-2321] - Detection of human SMG1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMG1 antibody NB100-2321 (lot 2) used for IP at 6 ug per reaction. SMG1 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated SMG1, NB100-2321 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: SMG1 Antibody [NB100-2321] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- SMG1 used at a dilution of 1:500 (2ug/ml). Detection: DAB