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- Antibody Data
- Antigen structure
- References [3]
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- Western blot [2]
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- Product number
- NB100-2320 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2320, RRID:AB_2239641
- Product name
- Rabbit Polyclonal SMG1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Tti1 and Tel2 are critical factors in mammalian target of rapamycin complex assembly.
Smg1 is required for embryogenesis and regulates diverse genes via alternative splicing coupled to nonsense-mediated mRNA decay.
Circadian oscillation of nucleotide excision repair in mammalian brain.
Kaizuka T, Hara T, Oshiro N, Kikkawa U, Yonezawa K, Takehana K, Iemura S, Natsume T, Mizushima N
The Journal of biological chemistry 2010 Jun 25;285(26):20109-16
The Journal of biological chemistry 2010 Jun 25;285(26):20109-16
Smg1 is required for embryogenesis and regulates diverse genes via alternative splicing coupled to nonsense-mediated mRNA decay.
McIlwain DR, Pan Q, Reilly PT, Elia AJ, McCracken S, Wakeham AC, Itie-Youten A, Blencowe BJ, Mak TW
Proceedings of the National Academy of Sciences of the United States of America 2010 Jul 6;107(27):12186-91
Proceedings of the National Academy of Sciences of the United States of America 2010 Jul 6;107(27):12186-91
Circadian oscillation of nucleotide excision repair in mammalian brain.
Kang TH, Reardon JT, Kemp M, Sancar A
Proceedings of the National Academy of Sciences of the United States of America 2009 Feb 24;106(8):2864-7
Proceedings of the National Academy of Sciences of the United States of America 2009 Feb 24;106(8):2864-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMG1 Antibody [NB100-2320] - Detection of Human SMG1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMG1 antibody NB100-2320 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMG1 Antibody [NB100-2320] - Detection of Human SMG1 on HeLa whole cell lysate using NB100-2320. IP of SMG1 was successful when using affinity purified rabbit anti-SMG1 antibodies NB100-2321 and another SMG1 antibody.
