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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-51406 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PHF14 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Immunogen sequence: AQKEGLLSEA AAEEDIADPF FAYCKQHADR LDRKWKRKNY LALQSYCKMS LQEREKQLSP EAQARINARL QQYRAKAELA RSTRPQAWVP REKLPRPLTS SASAIRKLMR KAELMGISTD IF Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.05 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Silencing expression of PHF14 in glioblastoma promotes apoptosis, mitigates proliferation and invasiveness via Wnt signal pathway.
Wu S, Luo C, Li F, Hameed NUF, Jin Q, Zhang J
Cancer cell international 2019;19:314
Cancer cell international 2019;19:314
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PHF14 in human colon using PHF14 Polyclonal Antibody (Product # PA5-51406) shows strong nuclear positivity in glandular cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1 PHF14 is overexpressed in glioblastoma multiforme (GBM) and lower PHF14 expression correlates with better outcome in GBM and LGG. a The cellular location of PHF14 confirmed by immunofluorescence (Scale bar = 50 mum). b Representative stainings of PHF14 in normal brain, grade II, grade III and grade IV glioma (Scale bar = 50 mum). c Correlation of the staining intensity of PHF14 with glioma grade. d - f Correlation of PHF14 mRNA expression with increasing WHO glioma grade according to REMBRANDT, TCGA and FRENCH data. g , h The relationship between PHF14 mRNA expression and molecular pathology of glioma. i , j Upregulation of PHF14 is a dismal prognostic factor in LGG by TCGA database. k Upregulation of PHF14 is a dismal prognostic factor in GBM by REMBRANDT database (*P < 0.05; **P = 0.01; ***P < 0.001; ****P < 0.0001)
