PA5-19940

antibody from Invitrogen Antibodies

Targeting: ACIN1 ACINUS, fSAP152, KIAA0670

Antibody data

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Validation data

Product number: PA5-19940 - Provider product page
Provider: Invitrogen Antibodies
Product name: Acinus Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: In Western blot applications, this antibody detects a band at ~220kDa. A suggested positive control is K562 cell lysate. PA5-19940 can be used with blocking peptide PEP-0065.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

ACIN1 protein structure - PA5-19940 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of K562 whole cell lysate using a Acinus polyclonal antibody (Product # PA5-19940) at 1 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Acinus in K562 whole cell lysate with Acinus Polyclonal Antibody (Product # PA5-19940) (IN) at 1 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot was performed using Anti-Acinus Polyclonal Antibody (Product # PA5-19940) and ~220 kDa, ~150 kDa, and ~75-80 kDa bands corresponding to splice forms of ACIN1 was observed across cell lines tested and downregulated upon cell treatments. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), untreated Jurkat (Lane 2), staurosporine treated Jurkat (Lane 3), camptothecin treated Jurkat (Lane 4), untreated U-2 OS (Lane 5), etoposide treated U-2 OS (Lane 6) and HeLa (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Downregulation of ACIN1 splice forms upon apoptotic stimuli are seen in both Jurkat and U-2 OS cells [doi: 10.1038/emboj.2009.106; 10.1038/43678]

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of K562 cells using a Acinus polyclonal antibody (Product # PA5-19940) at a 20 µg/mL dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry of Acinus in K562 cells with Acinus Polyclonal Antibody (Product # PA5-19940) at 5 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence of Acinus in K562 cells with Acinus Polyclonal Antibody (Product # PA5-19940) at 20 µg/mL. Green: Acinus Blue: DAPI staining

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence of Acinus in K562 cells with Acinus Polyclonal Antibody (Product # PA5-19940) at 20 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of ACIN1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Acinus Polyclonal Antibody (Product # PA5-19940) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2500), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear and cytoplasmic localisation for ACIN1 in HeLa. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. [doi: 10.1002/jcb.24893].