PA5-19948

antibody from Invitrogen Antibodies

Targeting: ACIN1 ACINUS, fSAP152, KIAA0670

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-19948

Provider

Invitrogen Antibodies

Product name

Acinus Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

In Western blot applications, this antibody detects a band at ~220kDa. A suggested positive control is K562 cell lysate. PA5-19948 can be used with blocking peptide PEP-0073.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 μg

Concentration

1 mg/mL

Storage

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of Acinus in K562 cells with Acinus Polyclonal Antibody (Product # PA5-19948) at 20 µg/mL. Green: Acinus Blue: DAPI staining

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of ACIN1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Acinus Polyclonal Antibody (Product # PA5-19948) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2500), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization for ACIN1. Panel e represents control HeLa cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of Acinus in K562 cells with Acinus Polyclonal Antibody (Product # PA5-19948) at 20 µg/mL. Green: Acinus Blue: DAPI staining

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of ACIN1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Acinus Polyclonal Antibody (Product # PA5-19948) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2500), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization for ACIN1. Panel e represents control HeLa cells with no primary antibody to assess background. The images were captured at 60X magnification.