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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-B5402 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ ENO3 / Enolase 3 Antibody (clone 5D1) LS-B5402
- Antibody type
- Monoclonal
- Description
- Purified from ascites by Protein A
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5D1
- Storage
- Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- ENO3 monoclonal antibody (M01), clone 5D1 Western blot of ENO3 expression in HeLa.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of ENO3 expression in transfected 293T cell line by ENO3 monoclonal antibody (M01), clone 5D1.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ENO3 is approximately 0.03 ng/ml as a capture antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ENO3 is approximately 0.03 ng/ml as a capture antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ENO3 is approximately 0.03 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-ENO3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
