MA5-29237

antibody from Invitrogen Antibodies

Targeting: ENO3

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-29237

Provider

Invitrogen Antibodies

Product name

ENO3 Monoclonal Antibody (01)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human ENO3/beta-enolase.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ENO3 in Lane A: HepG2 Whole Cell Lysate (30 µg), Lane B: Hela Whole Cell Lysate (30 µg), Lane C: MOLT4 Whole Cell Lysate (30 µg), Lane D: Raji Whole Cell Lysate (30 µg). Samples were probed using a ENO3 Monoclonal Antibody (Product # MA5-29237) at a 1:500 dilution, followed by a Goat Anti-Mouse IgG (H+L), Dylight 800 Secondary Antibody at a 1:150000 dilution. Western blot was performed under reducing conditions. Predicted band size:47 kDa. Observed band size:50 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ENO3 Monoclonal Antibody (1) (Product # MA5-29237) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: Hela Whole Cell Lysate, Lane C: MOLT4 Whole Cell Lysate, Lane D: Raji Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Mouse IgG H&L (DyLight™ 800) at 1:15,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 50 kDa.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of Human ENO3 expression on HeLa cells. The cells were treated according to manufacturer’s manual, stained with ENO3 Monoclonal Antibody (1) (Product # MA5-29237), then a FITC-conjugated Secondary antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.