Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-29239 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ENO3 Recombinant Rabbit Monoclonal Antibody (003)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire. This antibody has specificity for Human ENO3/beta-enolase.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 3
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human ENO3 in human skeletal muscle with ENO3 Recombinant Rabbit Monoclonal Antibody (3) (Product # MA5-29239, 1:200, formalin-fixed paraffin embedded sections).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ENO3 Immunoprecipitation using: Lane A: 0.5 mg Raji Whole Cell Lysate 2 µL with ENO3 Recombinant Rabbit Monoclonal Antibody (3) (Product # MA5-29239) and 15 µL of 50 % Protein G agarose. Primary antibody: ENO3 Recombinant Rabbit Monoclonal Antibody (3), at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 53 kDa.
