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LearnMA1-25833
antibody from Invitrogen Antibodies
Targeting: CLU
APOJ, CLI, CLU1, CLU2, KUB1, SGP-2, SP-40, TRPM-2
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- MA1-25833 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Apolipoprotein J Monoclonal Antibody (CLI-9)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA1-25833 detects Clusterin from human samples.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CLI-9
- Vial size
- 50 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Apolipoprotein J in transfected HEK 293T cells using an Apolipoprotein J monoclonal antibody (Product # MA1-25833).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Apolipoprotein J was achieved by transfecting HeLa cells with Apolipoprotein J specific siRNAs (Silencer® select Product # s3156, s3158). Western blot analysis (Fig. a) was performed using whole cell extracts from the HeLa knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Apolipoprotein J Mouse Monoclonal Antibody (Product # MA1-25833, 1 µg/mL) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Apolipoprotein J.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Apolipoprotein J Mouse Monoclonal Antibody (Product # MA1-25833) and 60 kDa band corresponding to Apolipoprotein J was observed across cell lines tested except Daudi and K-562. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Daudi (Lane 2) and K-562 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
