MA1-83879

antibody from Invitrogen Antibodies

Targeting: CLU APOJ, CLI, CLU1, CLU2, KUB1, SGP-2, SP-40, TRPM-2

Antibody data

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Validation data

Product number: MA1-83879 - Provider product page
Provider: Invitrogen Antibodies
Product name: Apolipoprotein J Monoclonal Antibody (3R3/2)
Antibody type: Monoclonal
Antigen: Other
Description: A suggested positive control for immunohistochemical applications is kidney from post streptococcal glomerulonephritis patients.
Antibody clone number: 3R3/2
Concentration: 1 mg/mL

CLU protein structure - MA1-83879 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Apolipoprotein J was achieved by transfecting HeLa cells with Apolipoprotein J specific siRNAs (Silencer® select Product # s3156, s3158). Western blot analysis (Fig. a) was performed using whole cell extracts from the HeLa knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Apolipoprotein J Mouse Monoclonal Antibody (Product # MA1-83879, 1 µg/mL) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Apolipoprotein J.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Apolipoprotein J Mouse Monoclonal Antibody (Product # MA1-83879) and 60 kDa band corresponding to Apolipoprotein J was observed across cell lines tested except LNCaP. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A549 (Lane 2), SW480 (Lane 3), LNCap (Lane 4), PC-3 (Lane 5) and K-562 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).