AF2937
antibody from Novus Biologicals
Targeting: CLU
APOJ, CLI, CLU1, CLU2, KUB1, SGP-2, SP-40, TRPM-2
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF2937 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Clusterin/APOJ Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Clusterin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant mouse Clusterin is observed and less than 5% cross-reactivity with recombinant rat Clusterin is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Semen clusterin is a novel DC-SIGN ligand.
Sabatte J, Faigle W, Ceballos A, Morelle W, Rodríguez Rodrígues C, Remes Lenicov F, Thépaut M, Fieschi F, Malchiodi E, Fernández M, Arenzana-Seisdedos F, Lortat-Jacob H, Michalski JC, Geffner J, Amigorena S
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 15;187(10):5299-309
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 15;187(10):5299-309
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Clusterin by Western Blot. Western blot shows lysates of human liver tissue and human serum. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Clusterin Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2937) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Clusterin Precusor at approximately 60-65 kDa and Clusterin alpha and beta chains at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Clusterin in Human Alzheimer's Disease Brain. Clusterin was detected in immersion fixed paraffin-embedded of human Alzheimer's disease brain using Human Clusterin Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2937) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.