PA5-18421

CLU antibody from Invitrogen Antibodies

APOJ, CLI, CLU1, CLU2, KUB1, SGP-2, SP-40, TRPM-2

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Data presented on provider website

Immunohistochemistry

Supportive data in Antibodypedia

Other assay

Supportive data in Antibodypedia

Provider product page for PA5-18421

Antibody Data

Product number

PA5-18421

Provider

Invitrogen Antibodies

Product name

Anti-Apolipoprotein J Polyclonal Antibody

Provider product page

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with canine based on sequence homology.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot staining of Human colon lysate using Product # PA5-18421 at a concentration of 0.3 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Apolipoprotein J by a Apolipoprotein J monoclonal antibody (Product # PA5-18421) at a concentration of 0.1 µg/mL. Human Colon lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Apolipoprotein J in Human Testis using a Apolipoprotein J monoclonal antibody (Product #PA5-18421) at 5 µg/mL. The Human Testis tissue section was paraffin embeded and detected using steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Apolipoprotein J in HeLa cells using a Apolipoprotein J monoclonal antibody (Product # PA5-18421) at 10 µg/mL for1hr. The cells were paraformaldehyde fixed and permeabilized with 0.15% Triton. Primary incubation was followed by Alexa Fluor 488 secondary antibody (2 µg/mL) showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL)followed by Alexa Fluor 488 secondary antibody (2 µg/mL).